抗小鼠CD3单抗(克隆号17A2) ，体内实验级重组，大鼠IgG2b Kappa | 悉得（Syd Labs）PA007199.r2b
体内实验级重组抗小鼠CD3单克隆抗体，大鼠IgG2b Kappa（克隆号17A2，货号：PA007199.r2b）是用哺乳动物细胞生产的重组抗体，适用于体外和体内研究，纯度>95%。其不变区为大鼠IgG2b kappa (rIgG2b或r2b)，可与重组大鼠IgG2b同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
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| 货号 | PA007199.r2b |
|---|---|
| 产品名称 | 抗小鼠CD3单抗(克隆号17A2) ，体内实验级重组，大鼠IgG2b Kappa | 悉得（Syd Labs）PA007199.r2b |
| 英文名 | In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Rat IgG2b Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | 分化簇3，CD3D，CD3E，CD3G |
| 概述 | 悉得（Syd Labs）提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | 17A2 |
| 同种型 | 大鼠 IgG2b Kappa |
| 应用 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受小鼠CD3蛋白影响的生物学途径的测定。 |
| 免疫源 | 抗小鼠CD3单克隆抗体(克隆号: 17A2)是用哺乳动物细胞生产的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质。 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD3单克隆抗体，大鼠IgG2b Kappa(克隆号17A2)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007199.r2b 悉得（Syd Labs）提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | 悉得（Syd Labs）在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系悉得（Syd Labs）中国代理商。 关于悉得（Syd Labs）产品如果有任何技术或其它问题，欢迎随时联系悉得（Syd Labs）国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
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抗小鼠CD3抗体(clone 17A2)参考文献:
1. CD3 aptamers promote expansion and persistence of tumor-reactive T cells for adoptive T cell therapy in cancer
Ashwathi Puravankara Menon,et al.Mol Ther Nucleic Acids. 2024.PMCID: PMC11091522
“The CD3/T cell receptor (TCR) complex is responsible for antigen-specific pathogen recognition by T cells, and initiates the signaling cascade necessary for activation of effector functions. CD3 agonistic antibodies are commonly used to expand T lymphocytes in a wide range of clinical applications, including in adoptive T cell therapy for cancer patients. A major drawback of expanding T cell populations ex vivo using CD3 agonistic antibodies is that they expand and activate T cells independent of their TCR antigen specificity. Therapeutic agents that facilitate expansion of T cells in an antigen-specific manner and reduce their threshold of T cell activation are therefore of great interest for adoptive T cell therapy protocols. To identify CD3-specific T cell agonists, several RNA aptamers were selected against CD3 using Systematic Evolution of Ligands by EXponential enrichment combined with high-throughput sequencing. The extent and specificity of aptamer binding to target CD3 were assessed through surface plasma resonance, P32 double-filter assays, and flow cytometry. Aptamer-mediated modulation of the threshold of T cell activation was observed in vitro and in preclinical transgenic TCR mouse models. The aptamers improved efficacy and persistence of adoptive T cell therapy by low-affinity TCR-reactive T lymphocytes in melanoma-bearing mice. Thus, CD3-specific aptamers can be applied as therapeutic agents which facilitate the expansion of tumor-reactive T lymphocytes while conserving their tumor specificity. Furthermore, selected CD3 aptamers also exhibit cross-reactivity to human CD3, expanding their potential for clinical translation and application in the future.”
2. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sunao Sugita,et al.Mol Arthritis Res Ther. 2017.PMCID: PMC5526238
“Background：A novel anti-mouse CD3ε antibody, Dow2, recognizes mouse CD3ε without activating T cells and suppresses T-cell activation. The purpose of this study was to determine whether Dow2 can inhibit T cells in uveitis.Methods：Experimental autoimmune uveitis (EAU) was induced in mice by immunization with retinal peptides, followed by administration of Dow2. Inflammation was evaluated by color fundus photography, optical coherence tomography, fluorescein angiography, and histology. Intraocular cells from EAU mice were used to examine the effect of Dow2 on retinal antigen-specific T cells. The effects of Dow2, conventional CD3ε antibodies, and isotype control immunoglobulin G (IgG) on splenic T cells were compared by assessing cell proliferation by the mixed lymphocyte reaction assay, inflammatory cytokine production by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and gene expression by quantitative reverse-transcription polymerase chain reaction (RT-PCR). T-cell subpopulations were characterized by flow cytometry to evaluate the expression of CD4, CD8, CD44, CD62L, and Foxp3.Results：Dow2 significantly reduced T-cell activation and counteracted activation associated with anti-CD3ε antibodies. Unlike conventional CD3ε antibodies, Dow2 treatment did not upregulate T helper (Th)1-/Th17-associated gene expression and cytokine production in splenic T cells. Interferon (IFN)-γ production by retinal antigen-specific T cells was also significantly reduced. Ocular inflammation was significantly reduced in Dow2-treated EAU mice compared to control EAU mice, with fewer T cells infiltrating into the retinas of Dow2-treated EAU mice. In immunohistochemistry, Th1 and Th17 cells invaded the retina in control EAU mice but not Dow2-treated EAU mice. No effects on peripheral T-cell numbers were observed following systemic administration of Dow2.Conclusion：The novel anti-CD3 antibody Dow2 can inhibit T cell-mediated inflammation in uveitis models. Thus, inhibition of T-cell activation by anti-CD3 therapy with this new antibody may protect uveitis patients from severe ocular inflammation.”
3. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Ranferi Ocaña-Guzmán,et al.Cells. 2022.PMCID: PMC9139304
“In recent years, a growing body of evidence has shown the presence of a subpopulation of macrophages that express CD3, especially in the context of mycobacterial infections. Despite these findings, the function of these cells has been poorly understood. Furthermore, the low frequency of CD3+ macrophages in humans limits the study of this subpopulation. This work aimed to evaluate the expression of CD3 in a murine macrophage cell line and its potential for the study of CD3 signaling. The murine macrophage cell line RAW was used to evaluate CD3 expression at the transcriptional and protein levels and the effect of in vitro infection with the Mycobacterium bovis Bacillus Calmette-Guérin (BCG) on these. Our data showed that RAW macrophages express CD3, both the ε and ζ chains, and it is further increased at the transcriptional level after BCG infection. Furthermore, our data suggest that CD3 can be found on the cell surface and intracellularly. However, this molecule is internalized constantly, mainly after activation with anti-CD3 stimulus, but interestingly, it is stably maintained at the transcriptional level. Finally, signaling proteins such as NFAT1, c-Jun, and IKK-α are highly expressed in RAW macrophages. They may play a role in the CD3-controlled signaling pathway to deliver inflammatory cytokines such as TNF and IL-6. Our study provides evidence to support that RAW cells are a suitable model to study the function and signaling of the CD3 complex in myeloid cells.”
悉得（Syd Labs）抗小鼠CD3单克隆抗体(克隆号17A2) （货号：PA007199.r2b）推荐同型对照抗体:
重组大鼠IgG2b同型对照抗体，体内实验级（In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody）
悉得（Syd Labs）相关重组IgG同型对照抗体（Recombinant IgG Reference Antibodies）:
重组小鼠IgG2a同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants）
重组小鼠IgG2b同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants）
重组小鼠IgG2c同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants）
悉得（Syd Labs）提供以下体内实验级重组抗小鼠CD3单克隆抗体（In Vivo Grade Recombinant Anti-Mouse CD3 Monoclonal Antibodies）:
体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，大鼠IgG2b Kappa（In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2)）
体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2b Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2b Kappa）
体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2a Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2a Kappa）
体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2c Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2c Kappa）
悉得（Syd Labs）提供以下抗人CD3抗体（anti-human CD3 antibodies）:
Muromonab生物类似药，科研级，抗人CD3单克隆抗体(克隆号: OKT3)（Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)）
Teplizumab 生物类似药，科研级，抗人CD3单克隆抗体(克隆号: OKT3)（Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)）
Foralumab 生物类似药，科研级，抗人CD3单克隆抗体(克隆号: OKT3)（Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)）
抗人 CD3单克隆抗体(克隆号: OKT3)（Anti-human CD3 monoclonal antibody (Clone: OKT3)）
抗人 CD3单克隆抗体(克隆号: SP34-2)（Anti-human CD3 monoclonal antibody (Clone: SP34-2)）
抗人 CD3单克隆抗体(克隆号: UCHT1)（Anti-human CD3 monoclonal antibody (Clone: UCHT1)）
悉得（Syd Labs）还提供以下抗小鼠CD3抗体（anti-mouse CD3 antibodies）:
抗小鼠 CD3e 单克隆抗体(克隆号: 145-2C11)（Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)）
抗小鼠 CD3e 单克隆抗体(克隆号: 500A2)（Anti-mouse CD3e monoclonal antibody (Clone: 500A2)）
抗小鼠 CD3单克隆抗体(克隆号: 17A2)（Anti-mouse CD3 monoclonal antibody (Clone: 17A2)）
请记住我们的产品信息: 抗小鼠CD3单克隆抗体(克隆号17A2)，体内实验级重组，大鼠IgG2b Kappa: PA007199.r2b 悉得（Syd Labs）In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa。