重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2b
Syd Labs (货号：PA007199.m2b）体内实验级重组抗小鼠CD3单抗(克隆号17A2)，小鼠IgG2b Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2b Kappa）。同种型: Mouse IgG2b Kappa；纯度: >95%
产品参数
| 货号 | PA007199.m2b |
|---|---|
| 产品名称 | 重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2b |
| 英文名 | In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2b Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇3，CD3D，CD3E，CD3G（cluster of differentiation 3, CD3D, CD3E, CD3G） |
| 概述 | Syd Labs提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定（Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.）。 |
| 克隆号 | 17A2 |
| 同种型 | Mouse IgG2b Kappa |
| 免疫源 | 抗小鼠CD3单克隆抗体(克隆号: 17A2)是在哺乳动物细胞中产生的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2b Kappa（In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2b Kappa)。 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2b Kappa（In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2b Kappa)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007199.m2b Syd Labs提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定（Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator）。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受小鼠CD3蛋白影响的生物学途径的测定。 |
文献
PA007199.m2b: Syd Labs抗小鼠CD3单克隆抗体(克隆号17A2) ，体内实验级重组，小鼠IgG2b Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2b Kappa）
抗小鼠CD3单克隆抗体(克隆号17A2)体内实验级重组小鼠IgG2b Kappa是免疫学与生物医药研究中极具价值的工具试剂。CD3分子作为T细胞受体（TCR）复合物的关键信号转导亚基，普遍表达于成熟T细胞表面。该抗体采用重组表达技术构建，将经典克隆号17A2的抗原结合区与小鼠IgG2b Kappa恒定区进行工程化重组，不仅严格保留了对小鼠CD3ε/γ/δ亚基的高特异性识别能力，还避免了异源宿主抗体在小鼠体内诱导的免疫原性响应。作为体内实验级重组试剂，产品在生产过程中经严格纯化与质控，确保极低内毒素及无载体蛋白添加，非常适合用于在体（in vivo）条件下精准调控T细胞功能。
在应用场景上，抗小鼠CD3单克隆抗体(克隆号17A2)体内实验级重组小鼠IgG2b Kappa常被用于小鼠模型的T细胞体内活化、增殖诱导以及免疫耐受与自身免疫性疾病的机制探究。由于采用了小鼠IgG2b Kappa亚型设计，该抗体能与同源小鼠宿主内的Fc受体更加稳定且可控地结合，从而在肿瘤免疫治疗评估、移植排斥反应控制以及体内免疫细胞表型分析等研究中提供高重现性的实验数据。对于高标准的in vivo功能实验而言，这种兼具17A2克隆号特异性与体内实验级重组高纯度特征的抗小鼠CD3单克隆抗体，是保障科研数据真实性与稳定性的重要基础。
抗小鼠 CD3 单克隆抗体（克隆号：17A2）参考文献：
Menon, A. P., et al. Mol Ther Nucleic Acids. 2024 May;35(2):102198. PMID: 38745854
“The CD3/T cell receptor (TCR) complex is responsible for antigen-specific pathogen recognition by T cells, and initiates the signaling cascade necessary for activation of effector functions. CD3 agonistic antibodies are commonly used to expand T lymphocytes in a wide range of clinical applications, including in adoptive T cell therapy for cancer patients. […] The aptamers improved efficacy and persistence of adoptive T cell therapy by low-affinity TCR-reactive T lymphocytes in melanoma-bearing mice.”
2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Castro, K., et al. Cells. 2022 May 13;11(10):1635. PMID: 35626672
“In recent years, a growing body of evidence has shown the presence of a subpopulation of macrophages that express CD3, especially in the context of mycobacterial infections. […] This work aimed to evaluate the expression of CD3 in a murine macrophage cell line and its potential for the study of CD3 signaling. […] Our study provides evidence to support that RAW cells are a suitable model to study the function and signaling of the CD3 complex in myeloid cells.”
3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H. D., Radbruch, A., et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. PMID: 34910301
“The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical diagnostics and specific subsets of immune cells.”
4. Rigid crosslinking of the CD3 complex leads to superior T cell stimulation
Nelson, A. D., Wang, L., et al. Front Immunol. 2024;15:1406456. PMID: 39281668
“Functionally bivalent non-covalent Fab dimers (Bi-Fabs) specific for the TCR/CD3 complex promote CD3 signaling on T cells. While comparing functional responses to stimulation with Bi-Fab, F(ab’)2 or mAb specific for the same CD3 epitope, we observed fratricide requiring anti-CD3 clustering. […] Thus, increasing rigidity of Fab-CD3 crosslinking between opposing effector-target pairs may result in stronger T cell effector function.”
5. Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival
Shirali, A. S., et al. J Clin Invest. 2018 Nov 1;128(11):4943-4956. PMID: 30277476
“The targeted delivery of therapeutic drugs to lymph nodes (LNs) provides an unprecedented opportunity to improve the outcomes of transplantation and immune-mediated diseases. […] Here, we have synthesized a nanoparticle (NP) as a carrier of anti-CD3, and optimized the conjugation strategy to coat the NP surface with MECA79 mAb (MECA79-anti-CD3-NP) to enhance LN accumulation. […] Short-term treatment of murine cardiac allograft recipients with MECA79-anti-CD3-NP resulted in significantly prolonged allograft survival in comparison with the control groups.”
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