D-luciferin（D-荧光素钾盐）使用说明是什么？

D-luciferin使用说明是什么？这些文献带你了解

D-luciferin（D-荧光素钾盐,CAS号:115144-35-9）使用说明是什么？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），作为一项经过国际市场检验、主要用于动物体内实验的产品，在品质与成本效益上具有双重优势，这表明其在国际市场上具有较大的竞争力。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现已强势登陆中国市场，期待为中国高校的动物实验中心和实验室注入新的活力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）凭借其卓越性能，获得了欧美众多大学core facilities的高度认可。正因如此，Syd Labs D-荧光素钾盐(D-Luciferin Potassium Salt)成为了许多国际知名学术期刊中的实验研究材料。

下面我们一起来看看涉及Syd Labs D-luciferin potassium salt(CAS号：115144-35-9) 的两篇引用文献，看看在具体的实验中如何使用D-荧光素钾盐：

55.

Th17 cells promote tumor growth in an immunocompetent orthotopic mouse model of prostate cancer

Zhenling Duan,et al.

Am J Clin Exp Urol. 2019 Aug 25;7(4):249-261. eCollection 2019.

Starting 3 or 7 days post-surgery, tumor growth was assessed using IVIS Lumina XRMS In Vivo Imaging System (PerkinElmer, Waltham, MA) as described previously [23]. Prior to imaging, the instrument was set to collect a minimum of 20,000 photon counts on the auto settings. Imaging parameters were prioritized as exposure time, binning, and ƒ stop. D-luciferin potassium salt (catalogue #MB000102-R70170, Syd Labs Inc., Natick, MA) was resuspended in PBS to a final concentration of 20 mg/ml. Mice were dosed with 10 μL/g luciferin substrate intraperitoneally and waited for 10 minutes to allow for distribution of substrate prior to image acquisition. Animals were anaesthetized with 3% isoflurane with a 1 L/min induction chamber flow rate and maintained at 2% isoflurane at a 0.5 L/min imaging chamber flow rate. After the completion of imaging, mice were replaced in the cage in a sternal position and observed for 10-15 minutes until becoming conscious. Endpoint was determined in consulting with a veterinarian, including the following criteria: palpable tumor in the lower abdomen > 1.5 cm in diameter, inability to eat or drink, inability to ambulate (i.e. when animal failed to move when approached), inability to pass urine, loss of 15% body weight during the course of weekly weighing, or loss of bioluminescent signals on imaging. If an animal met any of the criteria, it would be euthanized for endpoint analysis. Prior to euthanasia, in vivo imaging was performed as described above. The animal was weighed intact. The entire genitourinary (GU) bloc was dissected out of the carcass, fixed in 4% paraformaldehyde overnight at 4°C, and subsequently embedded in paraffin.

56.

Tumor-responsive, multifunctional CAR-NK cells cooperate with impaired autophagy to infiltrate and target glioblastoma

Jiao Wang,et al.

Chloroquine diphosphate salt (CQ) (98%) and adenosine 5’-monophosphate sodium salt hydrate (AMP) (99%) were purchased from ACROS Organics™. Hydrocortisone (98%) was purchased from Alfa Aesar. Ascorbic acid, Sodium chloride (NaCl) and Bovine serum albumin (BSA) were purchased from Thermo Fisher Scientific. Potassium chloride (KCl), Magnesium chloride (MgCl2), Sodium bicarbonate (NaHCO3), Adenosine (≥99%), DEAE-Dextran hydrochloride and Glucose were purchased from Sigma. Brefeldin A Solution (1000×) and Monensin Solution (1000×) were purchased from BioLegend. D-Luciferin Potassium Salt (>99%) was purchased from Syd Labs. LY294002, BAY11-782 and SP600125 were purchased from Cayman Chemical. Fetal bovine serum (FBS) and dimethyl sulfoxide (DMSO) were purchased from Corning. Recombinant human interleukin-12 (IL-2) was gifted from Akron Biotech. Recombinant human interleukin-15 (IL-15) and Fibroblast Growth Factor-basic (bFGF) were purchased from GoldBio. Recombinant Human RANTES (CCL5) was purchased from PeproTech. Recombinant human CXCL10, Recombinant human u-Plasminogen Activator/Urokinase, CF (uPA) and Recombinant human NKG2D/CD314 Fc Chimera were purchased from R&D systems. Biotin-Protein L was purchased from GenScript. BsiWI was purchased from New England Biolabs. RPMI1640, DMEM, IMDM, penicillin/streptomycin solution 100× (PS), 2-mercaptoethanol (50 mM), HEPES (1 M), chemically defined lipid concentrate, trypan blue solution and trypsin-EDTA were purchased from Gibco. Opti-MEM Reduced Serum Media was purchased from Invitrogen. EBM-2 was purchased from Lonza. Human AB serum was purchased from Valley Biomedical. Collagen I, rat tail was purchased from Enzo Life Sciences, Inc. RIPA lysis buffer system (sc-24948) was purchased from Santa Cruz Biotechnology

57.

GTP signaling links metabolism, DNA repair, and responses to genotoxic stress

Weihua Zhou,et al.

Cancer Discov. 2024.

To assess the bioluminescence of brain tumors, mice were treated with 150 mg/kg D-luciferin (Cat# MB000102-R70170, Syd labs) by intraperitoneal injection and then imaged 10 min later using an IVIS™ Spectrum imaging system (PerkinElmer).

58.

Humanized anti-CD11d monoclonal antibodies suitable for basic research and therapeutic applications

Eoin N Blythe,et al.

Antib Ther. 2024.

Next, all the media were removed, replaced with 100 μl of RMPI complete media containing 150 μg/ml D-luciferin (Syd Labs), and treated with various conditions.

59.

Phosphoproteomic Profiling Reveals mTOR Signaling in Sustaining Macrophage Phagocytosis of Cancer Cells

Bixin Wang,et al.

Cancers (Basel) 2024.

Commercial reagents were obtained from the following suppliers: fetal bovine serum (FBS; Omega Scientific, Tarzana, CA, USA; #FB-02); cell culture media and additives (Gibco, Thermo Fisher Scientific, Waltham, MA, USA); penicillin/streptomycin (GenClone, Genesee Scientific, San Diego, USA; #25-512); murine macrophage colony-stimulating factor (M-CSF; Thermo Fisher Scientific, Waltham, MA, USA; #315-02-100UG); InVivoMAb anti-human CD47 B6H12 (Bio X Cell, Lebanon, PA, USA; #BE0019-1); rapamycin (LC Laboratories, Woburn, USA; #NC9362949); temsirolimus (Cayman Chemical, Ann Arbor, USA; #11590); D-Luciferin (Syd Labs, Beverly, CA, USA, #MB000102-R70170); TrypLE Express (Gibco, Thermo Fisher Scientific, Waltham, MA, USA; #12604-021); Sytox Blue buffer (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; #S34857); 10× PBS(Corning, Corning, NY, USA; #46-103-CM); Annexin V binding buffer (BD Biosciences, San Jose, CA, USA; #556454); human serum (Omega Scientific, Tarzana, USA; #HS-20); LysoTracker (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; #L7528); CellTrace CFSE (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; #C34554); and CellTrace Far Red (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; #C34564).