重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2c
Syd Labs (货号：PA007199.m2c）体内实验级重组抗小鼠CD3单抗(克隆号17A2)，小鼠IgG2c Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2c Kappa）。同种型: Mouse IgG2c Kappa；纯度: >95%
产品参数
| 货号 | PA007199.m2c |
|---|---|
| 产品名称 | 重组抗小鼠CD3单抗(17A2) | Syd Labs PA007199.m2c |
| 英文名 | In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2c Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇3，CD3D，CD3E，CD3G（cluster of differentiation 3, CD3D, CD3E, CD3G） |
| 概述 | Syd Labs提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定（Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.）。 |
| 克隆号 | 17A2 |
| 同种型 | Mouse IgG2c Kappa |
| 免疫源 | 抗小鼠CD3单克隆抗体(克隆号: 17A2)是在哺乳动物细胞中产生的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质。提供特级体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2c Kappa（In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2c Kappa)。 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CD3单克隆抗体(克隆号17A2)，小鼠IgG2c Kappa（In Vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody（Clone 17A2）,Mouse IgG2c Kappa)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007199.m2c Syd Labs提供重组大鼠 IgG2b同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定（Recombinant rat IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator）。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受小鼠CD3蛋白影响的生物学途径的测定。 |
文献
PA007199.m2c: Syd Labs抗小鼠CD3单克隆抗体(克隆号17A2) ，体内实验级重组，小鼠IgG2c Kappa（In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2c Kappa）
抗小鼠CD3单克隆抗体（克隆号17A2，体内实验级重组，小鼠IgG2c Kappa）是免疫学研究与肿瘤免疫治疗评估中的关键靶向工具。CD3分子（包含CD3D、CD3E、CD3G链）作为T细胞受体（TCR）复合体的核心组分，在T细胞激活、信号转导及细胞毒性反应中发挥决定性作用。克隆号17A2能特异性识别小鼠CD3复合体，常用于T细胞耗竭、T细胞激活以及体外/体内免疫应答调控研究。重组生产的小鼠IgG2c Kappa亚型不仅保持了高度的批次间稳定性，更有效降低了异源蛋白引发的免疫原性风险。
在体内实验（In Vivo Studies）中，选择体内实验级重组抗小鼠CD3单克隆抗体（克隆号17A2，小鼠IgG2c Kappa）能够显著提升实验数据的可靠性与重复性。体内实验级抗体具备超低内毒素、无载体蛋白及无防腐剂等特点，可有效避免非特异性炎症干扰与小鼠系统性毒性反应。作为成熟的抗小鼠CD3单克隆抗体，克隆号17A2结合小鼠IgG2c Kappa骨架的独特结合特性，使其在小鼠肿瘤模型、自身免疫性疾病研究及T细胞功能分析中表现出卓越的靶向活性与生物学效应。
抗小鼠 CD3 单克隆抗体（克隆号：17A2）参考文献：
Menon, A. P., et al. Mol Ther Nucleic Acids. 2024 May;35(2):102198. PMID: 38745854
“The CD3/T cell receptor (TCR) complex is responsible for antigen-specific pathogen recognition by T cells, and initiates the signaling cascade necessary for activation of effector functions. CD3 agonistic antibodies are commonly used to expand T lymphocytes in a wide range of clinical applications, including in adoptive T cell therapy for cancer patients. […] The aptamers improved efficacy and persistence of adoptive T cell therapy by low-affinity TCR-reactive T lymphocytes in melanoma-bearing mice.”
2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Castro, K., et al. Cells. 2022 May 13;11(10):1635. PMID: 35626672
“In recent years, a growing body of evidence has shown the presence of a subpopulation of macrophages that express CD3, especially in the context of mycobacterial infections. […] This work aimed to evaluate the expression of CD3 in a murine macrophage cell line and its potential for the study of CD3 signaling. […] Our study provides evidence to support that RAW cells are a suitable model to study the function and signaling of the CD3 complex in myeloid cells.”
3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H. D., Radbruch, A., et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. PMID: 34910301
“The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical diagnostics and specific subsets of immune cells.”
4. Rigid crosslinking of the CD3 complex leads to superior T cell stimulation
Nelson, A. D., Wang, L., et al. Front Immunol. 2024;15:1406456. PMID: 39281668
“Functionally bivalent non-covalent Fab dimers (Bi-Fabs) specific for the TCR/CD3 complex promote CD3 signaling on T cells. While comparing functional responses to stimulation with Bi-Fab, F(ab’)2 or mAb specific for the same CD3 epitope, we observed fratricide requiring anti-CD3 clustering. […] Thus, increasing rigidity of Fab-CD3 crosslinking between opposing effector-target pairs may result in stronger T cell effector function.”
5. Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival
Shirali, A. S., et al. J Clin Invest. 2018 Nov 1;128(11):4943-4956. PMID: 30277476
“The targeted delivery of therapeutic drugs to lymph nodes (LNs) provides an unprecedented opportunity to improve the outcomes of transplantation and immune-mediated diseases. […] Here, we have synthesized a nanoparticle (NP) as a carrier of anti-CD3, and optimized the conjugation strategy to coat the NP surface with MECA79 mAb (MECA79-anti-CD3-NP) to enhance LN accumulation. […] Short-term treatment of murine cardiac allograft recipients with MECA79-anti-CD3-NP resulted in significantly prolonged allograft survival in comparison with the control groups.”
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