文献

抗人CD4单克隆抗体，体内实验级重组，小鼠IgG1 Kappa (克隆号13B8.2)（In Vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa，货号：PA007385.m1 Syd Labs）

重组 13B8.2 抗体可与人 CD4 蛋白（CD4 分子）结合，这是一种糖蛋白，也是 T 细胞受体（TCR）的共受体。CD4 蛋白表达于多种免疫细胞表面，如辅助性 T 细胞、单核细胞、巨噬细胞和树突状细胞。

抗人CD4单抗(13B8.2)部分引用文献:

1. Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs
Silvin, A., et al. Elife. 2020; 9:e57063. PMID: 33289628
“Peripheral blood mononuclear cells (PBMCs) from 12 donors were stained with anti-CD4–PE (clone 13B8.2) antibody, along with anti-CD27–PE-Cy5 (clone O323), anti-CD25–eFluor450 (clone BC96), anti-CD31–PE-Cy7 (clone WM59), and anti-CD45RA–FITC (clone JS-83) to identify recent thymic emigrants (RTEs), mature naive T cells, and naive Tregs in the CD4+ lineage. …The study explored the plasticity of Th2, Th1, Th17, and Treg cells, noting that Th2 cells can adopt a Th1-like phenotype, while Th1 and Th17 cells can transition to Th2 in helminth-infected mice, and Th17 cells can acquire regulatory features like IL-10 production under IL-12 or IL-27 influence.”

2. Macrophage infection via selective capture of HIV-1-infected CD4+ T cells
Baxter, A. E., et al. Cell Host Microbe. 2014 Dec 10;16(6):711-21. PMID: 25467409
“The study used the 13B8.2 antibody (anti-human CD4, 10 µg/ml) as an inhibitor of HIV-1 Env-receptor interactions, alongside soluble CD4, Tak-779, 2G12, and T20. …The results suggest that macrophage uptake of HIV-1-infected CD4+ T cells contributes to the macrophage reservoir and viral dissemination in vivo, particularly after transmission or during massive CD4+ T cell death in lymphoid tissues. …Detection of viral DNA or proteins in phagocytes may indicate uptake of infected immune cells rather than direct infection.”

3. In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas
Troadec, S., et al. J Immunother. 2007 Feb-Mar;30(2):190-202. PMID: 17273799
“A baculovirus-expressed chimeric recombinant IgG1 antibody (rIgG1) with human Cγ1 and Cκ domains, derived from the murine anti-CD4 monoclonal antibody 13B8.2, was tested for antitumoral activity against CD4+ T-cell lymphomas. …The 13B8.2 antibody, specific for the CDR3-like loop of CD4, inhibited HIV-1 replication and T cell proliferation in vitro and showed promising results in CD4+ lymphoma cell lines, leading to plans for in vivo preclinical studies in a murine xenograft model.”

4. Molecular and cellular analysis of human immunodeficiency virus-induced apoptosis in lymphoblastoid T-cell-line-expressing wild-type and mutated CD4 receptors
Guillerm, C., et al. J Virol. 1998 Oct;72(10):8061-72. PMID: 9733846
“The 13B8.2 monoclonal antibody, targeting the CD4 region critical for receptor dimerization, prevented apoptosis in HIV-infected cells without inhibiting viral replication. …The study highlighted high levels of HIV replication and immune system activation in vivo, contributing to enhanced apoptosis in both infected and bystander CD4 cells. …Fas signaling was noted as a potential contributor to T-cell depletion in HIV-1-infected patients.”

5. Differential CD4-dependent inhibition of JNK but not Erk-2 activities by anti-CD4 monoclonal antibodies
Chene, C., et al. Int Immunol. 1998 Jul;10(7):869-78. PMID: 9701025
“The study observed differential inhibition of JNK but not Erk-2 activities by the anti-CD4 monoclonal antibody 13B8.2 in Jurkat T cells. …In vivo experiments in mouse models showed that administration of 13B8.2 led to selective inhibition of JNK activity in T cells, demonstrating that CD4 ligation modulates T cell activation through differential kinase inhibition.”