重组抗人CD19单抗(FMC63)流式 | Syd Labs PA007422.h1Fs
Syd Labs非偶联重组抗人CD19单克隆抗体，人IgG1 Fc Silent Kappa（克隆号FMC63，货号：PA007422.h1Fs）是用哺乳动物细胞生产的重组抗体，适用于流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）等研究，纯度>95%。其不变区为人Human IgG1 kappa (hIgG1或h1)，可与重组人IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007422.h1Fs |
|---|---|
| 产品名称 | 重组抗人CD19单抗(FMC63)流式 | Syd Labs PA007422.h1Fs |
| 英文名 | Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: FMC63), Human IgG1 Fc Silent Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | B-淋巴细胞抗原 CD19，分化簇 19，B-淋巴细胞表面抗原 B4，T-细胞表面抗原 Leu-12，CVID3 |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | FMC63 |
| 同种型 | 人 IgG1 kappa |
| 特异性 | 该重组 FMC63 抗体与人 CD19 蛋白特异性结合 |
| 免疫源 | 重组抗人 CD19 单克隆抗体 (克隆：FMC63)在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 非偶联重组抗人CD19单克隆抗体，人IgG1 Fc Silent Kappa(克隆号FMC63) 用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007422.h1Fs Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
抗人CD19单克隆抗体(克隆号FMC63)，非偶联重组，人IgG1 Fc Silent Kappa（Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: FMC63), Human IgG1 Fc Silent Kappa，货号：PA007422.h1Fs Syd Labs）
FMC63抗体可特异性结合人CD19蛋白，这是一种表达于所有B细胞系（包括人浆细胞）的跨膜蛋白。CD19在人B细胞中发挥两个主要作用：作为接头蛋白将细胞质信号蛋白招募至细胞膜，以及在CD19/CD21复合体中发挥作用以降低B细胞受体信号通路的激活阈值。CD19是B淋巴细胞发育和淋巴瘤诊断的生物标志物，也可作为白血病免疫治疗的靶点。
抗人CD19单抗(FMC63)部分参考文献:
1. Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.
Müller, N., et al. Mol Ther Methods Clin Dev. 2022 Jul. doi: 10.1016/j.omtm.2022.06.004
“Cells were stained with FITC-conjugated anti-CD19 mAb (clone 1D3) and analyzed by flow cytometry to monitor B cell depletion. The depletion efficiency was >95% in peripheral blood as determined by flow cytometry using 1D3 antibody. Peripheral blood was collected and stained with anti-CD19 (1D3) and anti-CD3 antibodies. Flow cytometry was performed on a FACSCalibur using CellQuest software. B cell depletion was confirmed by the absence of CD19+ cells in flow analysis.”
2. Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus.
Ma, J., et al. Cell Mol Immunol. 2021 Aug. doi: 10.1038/s41423-020-0472-1
“For flow cytometry, cells were stained with anti-CD19 (clone 1D3) antibody to identify B cells. The percentage of CD19+ B cells was significantly reduced in treated mice. Spleen cells were isolated and stained with 1D3 antibody for CD19 expression. Data were acquired on a BD FACSCanto II flow cytometer. Treatment led to long-term B cell depletion as measured by flow.”
3. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells.
Kochenderfer, J. F., et al. Blood. 2010 Nov. doi: 10.1182/blood-2010-01-265041
“B cells were identified by staining with PE-conjugated anti-CD19 (clone 1D3) antibody. Flow cytometry analysis showed eradication of CD19+ cells in lymphoma. The antibody 1D3 was used to gate B cell population in bone marrow. Peripheral blood B cells were monitored weekly using 1D3. CAR-T cells specifically targeted CD19+ cells as confirmed by flow.”
4. Self-renewal of B-1 lymphocytes is dependent on CD19.Krop, I., et al. Eur J Immunol. 1996 Jan. doi: 10.1002/eji.1830260137
“Cells were stained with anti-CD19 mAb (1D3) for flow sorting. Peritoneal cells were analyzed for CD19 expression using 1D3. The antibody 1D3 was used in all flow cytometry experiments for B cell identification. Adoptive transfer experiments used sorted CD19+ cells with 1D3. B-1 cell self-renewal was assessed by limiting dilution analysis after sorting with 1D3.”
5. The signaling activity of murine CD19 is regulated during cell development.
Krop, I., et al. J Immunol. 1996 Jul. PMID: 8683154
“Murine B cells were stained with biotinylated 1D3 anti-CD19 mAb followed by streptavidin-PE. Flow cytometry was used to assess CD19 expression levels during development. The 1D3 clone was specific for murine CD19. Developmental stages were gated based on 1D3 staining intensity. Signaling was correlated with CD19 surface density measured by 1D3.”