Syd Labs D-荧光素钾盐配制方法是什么？

Syd Labs D-荧光素钾盐配制方法是什么？这些文献带你揭晓答案

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，CAS号:115144-35-9）配制方法是什么？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），作为全球市场验证的动物体内实验产品，以卓越的品质与颇具竞争力的价格脱颖而出。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现已登陆中国市场，旨在为中国高校的动物实验中心注入全新科研动力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170），凭借其卓越性能，已获得欧美多所大学core facilities的广泛好评。作为美国Syd Labs公司的特色产品之一，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）不仅在众多欧美实验室中被用于动物体内实验，而且被许多国际知名学术期刊大量引用，这充分说明Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在科研领域具有高度的实用价值。另外，值得一提的是，除了Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt），美国Syd Labs公司还提供一系列用于体内实验的重组单抗产品，同样适用于注射体内实验，是相关科研工作者的优选产品。

以下三篇文献体现了Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在生物科学领域的部分具体应用：

21.

Engineered natural killer cells impede the immunometabolic CD73-adenosine axis in solid tumors

Andrea M Chambers, Kyle B Lupo, Jiao Wang, Jingming Cao, Sagar Utturkar, Nadia Lanman, Victor Bernal-Crespo, Shadia Jalal, Sharon R Pine, Sandra Torregrosa-Allen, Bennett D Elzey, Sandro Matosevic

Elife. 2022 Jul 11:11:e73699. doi: 10.7554/eLife.73699.

A549 cells (2 × 106) cells were inoculated subcutaneously (SC) in the right flank of NRG mice (n=6–7/group) with 100 µL matrigel. PBS (100 µL) and engineered NK and non-engineered NK cells at 5 million cells/mouse were injected once a week via tail vein after the tumors were approximately 80 mm3. Also, 1 µg/mouse of IL-15 (Shenandoah Biotech) was injected three times per week with an intraperitoneal injection (ip). Tumor size was measured using the Spectral Ami Optical imaging system once per week. Mice were injected with 10 µL/g and 150 mg Luciferin/kg body weight with a stock solution of 15 mg/mL D-Luciferin (Syd Labs). The D-Luciferin was injected intra-peritoneally (i.p.) for 10 min before imaging. Body weights of the mice were also recorded during the treatment period. Tumor growth was monitored until mice met predefined endpoint criteria. Tumor tissues were processed in 10% neutral-buffered formalin for histological IHC analysis. For homing studies, NK cells were administered once per week with IL-2 three times per week, and NK cells were detected by IHC as described.

22.

Anti-tumor antibody isotype response can be modified with locally administered immunoadjuvants

Adam A Walters, Abrar Ali, Julie Tzu-Wen Wang, Khuloud T Al-Jamal

Drug Deliv Transl Res. 2023 Jul;13(7):2032-2040. doi: 10.1007/s13346-022-01258-8. Epub 2022 Nov 23.

Nunc Maxisorp™ flat-bottom plates (ELISA plates) and Bicinchoninic acid assay (BCA) were obtained from Thermo Fisher Scientific (UK). The secondary antibodies were horse radish peroxidase (HRP)-conjugated Goat anti-Mouse IgM, and Goat anti-Mouse IgG1 obtained from BIO-RAD, Anti-Mouse IgG HRP-linked antibody obtained from Cell Signalling Technology (UK) and Goat Anti-Mouse IgG2c-HRP antibody obtained from SouthernBiotech (USA). Bovine serum albumin (BSA) and ovalbumin (OVA) were obtained from Sigma-Aldrich (UK). Addavax was obtained from Invivogen (France). CpG 1668 (CpG) was synthesised by Eurogentec (Belgium). Trypsin EDTA, GlutaMAX™, RPMI 1640 media, phosphate buffer saline (PBS) and fetal calf serum (FCS) were obtained from Gibco, Thermo Fischer Scientific. Tetramethylbenzidine (TMB) high-sensitivity substrate solution and ELISA stop solution were obtained from BioLegend (USA). D-Luciferin potassium salt was obtained from Syd Labs (USA).

23.

Cancer immunotherapy based on image-guided STING activation by nucleotide nanocomplex-decorated ultrasound microbubbles

Xuefeng Li, Sina Khorsandi, Yifan Wang, Julien Santelli, Kristin Huntoon, Nhu Nguyen, Mingming Yang, DaeYong Lee, Yifei Lu, Ruoqi Gao, Betty Y S Kim, Caroline de Gracia Lux, Robert F Mattrey, Wen Jiang, Jacques Lux

Nat Nanotechnol. 2022 Aug;17(8):891-899. doi: 10.1038/s41565-022-01134-z. Epub 2022 May 30.

BALB/cJ mice were used to study the growth and metastasis of Luc-4T1 tumours in vivo as follows. At 12 days post tumour implantation, mice were intraperitoneally injected with 1.5 mg D-luciferin (Syd Labs) per 10 g body weight; at 10 min after injection, the presence and metastases of the tumours were monitored by bioluminescence imaging (BLI) using an In Vivo Imaging System (PerkinElmer IVIS® Lumina III). Data were analysed and presented with Aura Imaging software. All images were adjusted to the same color scale and display threshold. The IVIS images of the upper body were used to detect luminescence signals in the lung. After the live imaging, mice that met the criteria for early euthanasia were sacrificed36, and major organs including heart, liver, spleen, lung, kidneys, and tumour were collected for additional IVIS imaging to analyse luminescence signals in these organs.