Syd Labs D-荧光素钾盐发光原理是什么？

Syd Labs D-荧光素钾盐发光原理是什么？这些文献有所体现

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt,CAS号:115144-35-9）发光原理是什么？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），这款久经全球市场验证、由美国Syd Labs公司自主研发的优质产品主要被用于动物体内实验。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）无论是价格还是质量都具有很强的竞争优势，使其在海外市场赢得了广泛赞誉。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现正式进军中国，旨在为中国高校动物实验中心提供全新的科研动力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170），多年来被众多欧美大学的core facilities购买、使用和复购，积累了良好的口碑与信誉。除了被多所欧美实验室广泛应用于动物体内实验外，Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）还反复出现在国际权威学术期刊之中，被相关科研工作者频繁收录于文献之中，这彰显了其在生命科学领域的广泛适用性和实用性。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）在以下三篇文献中得到了具体应用，彰显了其在生物科学领域的实用价值：

36.

Circulating tumor cells exhibit metastatic tropism and reveal brain metastasis drivers

Remi Klotz , Amal Thomas, Teng Teng, Sung Min Han, Oihana Iriondo, Lin Li, Sara Restrepo-Vassalli, Alan Wang , Negeen Izadian, Matthew MacKay , Byoung-San Moon, Kevin J Liu , Sathish Kumar Ganesan , Grace Lee , Diane S Kang , Charlotte S Walmsley, Christopher Pinto, Michael F Press, Wange Lu, Janice Lu, Dejan Juric, Aditya Bardia, James Hicks, Bodour Salhia, Frank Attenello, Andrew D Smith, Min Yu

Cancer Discov. 2020 Jan;10(1):86-103. doi: 10.1158/2159-8290.CD-19-0384. Epub 2019 Oct 10.

All animal experiments were carried out in accordance with approved protocols from the Institutional Animal Care and Use Committee of USC. Metastatic tumors were established by inoculation of 1 × 105 GFP-LUC–labeled CTCs in 100 μL of PBS into the left cardiac ventricles of 6–8-week-old female NSG mice supplemented with subcutaneous slow-release estrogen pills. Metastasis formation was monitored every 2 weeks by in vivo imaging using IVIS Lumina III (PerkinElmer) following intraperitoneal injection of 100 μL of d-luciferin substrate (Syd Labs). Metastases were confirmed by ex vivo bioluminescence imaging and resected under sterile conditions. Brain lesions were placed in brain tumor dissociation medium (Miltenyi Biotec). Lung, ovary, and kidney lesions were minced and placed in dissociation medium containing RPMI supplemented with 2 mg/mL collagenase I and 15 U/mL DNAse. Samples were further dissociated into single-cell suspension by automated dissociation using the gentleMACS dissociator. Bone lesions were placed in tumor dissociation media (Miltenyi Biotec) and gently ground three times in a mortar. Bone tissues were further dissociated in tumor dissociation media on an orbital shaker at 37°C for 45 minutes. After dissociation, all tissues were filtered through a cell strainer (70 μm), and cells were washed twice in PBS, then resuspended in PBS with 1% BSA. GFP+ cells were sorted for further propagation in culture, inoculation in mice, or RNA-seq and ATAC-seq. GFP− cells were also sorted and used for RNA extraction.

37.

The fusion peptide proximal region of the HIV-1 envelope glycoproteins regulates exposure of immunogenic epitopes at the trimer base

Roberth Anthony Rojas Chávez,, Devlin Boyt , Changze Han, Li Wu, and Hillel Haim

bioRxiv preprint doi: https://doi.org/10.1101/2020.10.28.360131

Cf2Th-CD4+CCR5+ cells were seeded in 96-well luminometer-compatible plates at a density of 7.5 × 103 cells per well and infected the next day. For neutralization assays, virus preparations were incubated at 37°C in the absence or presence of mAbs or serum for 1 h.Samples were then added to Cf2Th-CD4+CCR5+ cells and incubated for 3 d to allow infection.To measure infection, the medium was removed, cells were lysed with 35 µl passive lysis buffer (Promega) and subjected to three freeze-thaw cycles. To measure luciferase activity, 100 µl of luciferin buffer (15 mM MgSO4, 15 mM KPO4 [pH 7.6], 1 mM ATP, and 1 mM dithiothreitol) and 50 µl of 1 mM D-luciferin potassium salt (Syd Labs, MA) were added to each sample.Luminescence was recorded using a Synergy H1 microplate reader (BioTek Instruments).

38.

Dormant senescence-prone cells (DSPC): a lifetime memory about systemic genotoxic stress

Katerina I. Leonova, Ilya I. Gitlin, Anatoli S. Gleiberman, Ilia Toshkov, Nicholas H.Trageser, Natalia Issaeva, Marina P. Antoch and Andrei V. Gudkov

research square doi: https://doi.org/10.21203/rs.3.rs-1844327/v1

Mice were injected intraperitoneally with a 100 µl solution of 30 mg/mL D-luciferin potassium salt(Syd Labs) in D-PBS without calcium and magnesium. At 5 minutes post-injection, isofluraneanesthetized mice were placed into the IVIS Spectrum in vivo bioluminescent imaging system(PerkinElmer) for detection of luciferase activity (60-second exposure). Bioluminescence in p16LUC mice was quantified as total flux (p/s) of luminescent signal from the abdomen using via Living Image® software.