SYBR Green Dye-Based One-Step qRT-PCR Kit

描述

MB128: Dye-Based One-Step qRT-PCR Kit

Background

The SYBR Green dye-based one-step qRT-PCR kit is ready-to-use 2x concentrated solution for quantitative, reverse transcription-qPCR. Except DNA template and primers, the 2x SYBR Green dye-based one-step qRT-PCR master mix contains all other components for RT and qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, 4G Reverse Transcriptases, murine RNase inhibitor, heat-labile UDG, and antibody-based hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x SYBR Green dye-based one-step qRT-PCR master mix to specific primers, DNA template, 50x ROX reference dye 1 and 2, and water.

The SYBR Green dye-based one-step qRT-PCR kit is specially designed for SYBR Green I-based qPCR detection using RNA (e.g. SARS-COV-2 virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit integrates the superior performance of 4G Reverse Transcriptase and antibody-based hot start Taq DNA Polymerase with optimized buffer, which enables the detection sensitivity of one step qRT-PCR SYBR green kit to reach 1 pg total RNA. The enhancement factor added in the buffer system can effectively reduce the formation of primer dimer and improve the specificity of products. This kit provides a convenient master mix form for easy and quick operation.

Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features of the Dye-Based One-Step qRT-PCR Kit:
* Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination.
* High detection sensitivity of one step qRT-PCR SYBR green kit: 1 pg total RNA.
* More stable amplification curve with low concentration template.
* Reduced formation of primer dimers and improved specificity of qPCR products.
* Convenient, easy, and quick operation.

Components of the Dye-Based One-Step qRT-PCR Kit:
One-step SYBR Green enzyme mix: 4G Reverse Transcriptases, murine RNase inhibitor, and antibody-based hot start Taq DNA polymerase.
One-step SYBR Green buffer mix (2X): Buffer optimized for reverse transcription-qPCR; Mixtures of dNTP mix, MgCl2, specific enhancer factors, and SYBR Green I.
ROX reference dye 1 (50X).
ROX reference dye 2 (50X).
RNase-free H2O.

Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

Please remember our product information: SYBR Green Dye-Based One-Step qRT-PCR Kit: MB128 Syd Labs