Recombinant Human VEGF Protein
Syd Labs Recombinant Human VEGF Protein BP000027-GD1, The activity of the recombinant human VEGF 165 protein is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/mL.
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| 货号 | BP000027-GD1 |
|---|---|
| 产品名称 | Recombinant Human VEGF Protein |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Vascular Endothelial Growth Factor Isoform 165, Vascular Endothelial Growth Factor |
| 来源 | HEK 293 cell-derived. Ala27-Arg191, Accession # NP_001165097 |
| 形式 | Lyophilized from a 0.2 um filtered solution with BSA as a carrier protein. |
| 纯度 | > 95% by SDS-PAGE under reducing conditions. |
| 内毒素 | < 1.0 EU per ug of the protein by the LAL method. |
| 运输 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal VEGF / VEGF-A / VPF Protein performance should be determined experimentally by the investigator. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000027-GD1: Recombinant Human VEGF165 (aa 27-191) Protein
Background
Human VEGF-A, also known as vascular endothelial growth factor (VEGF) and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. There are 8 differently spliced isoforms of VEGF, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF-A stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
Source: HEK 293 cell-derived. Ala27-Arg191, Accession # NP_001165097
N-terminal sequence analysis: Met-Asn.
Structure/Form: Disulfidelinked homodimer.
Predicted molecular mass: 19.2 kDa (monomer).
SDS-PAGE: 17-20 kDa, reducing conditions.
The VEGF activity is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/ml.
Endotoxin: < 1.0 EU per ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions.
Formulation: Lyophilized from a 0.2 um filtered solution with BSA as a carrier protein.
Reconstitution: Reconstitute at 50 µl ddH2O to the concentration of 1.0 mg/ml. To further dilute the VEGF concentration under 10 µg/ml, 1% BSA is recommended..
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other VEGF Proteins:
Recombinant human VEGF-B Protein
Recombinant human VEGF-C Protein
Recombinant human VEGF-D Protein
Recombinant human PGF(Placenta Growth Factor)
Recombinant human VEGF121 Protein
VEGF-related Antibodies:
VEGF-A polyclonal antibody
VEGF-B polyclonal antibody
VEGF-D polyclonal antibody
VEGFR2 monoclonal antibody
VEGFR2 (Phospho-Tyr951) polyclonal antibody
VEGFR2 (Phospho-Tyr1214) polyclonal antibody
VEGFR2 (Phospho-Tyr1175) polyclonal antibody
VEGFR2 (Phospho-Tyr1059) polyclonal antibody
VEGFR2 (Phospho-Tyr1054) polyclonal antibody
VEGFR2 polyclonal antibody
VEGFR1 polyclonal antibody
VEGFR1 (Phospho-Tyr1333) polyclonal antibody
VEGFR1 (Phospho-Tyr1048) polyclonal antibody
VEGF-related ELISA Kits:
Human VEGF-A ELISA Kit
Rat VEGF-A ELISA Kit
Mouse VEGF-A ELISA Kit
Human EG-VEGF ELISA Kit
Human VEGFR1 ELISA Kit
Human VEGF-C ELISA Kit
Please remember our product information: Recombinant Human VEGF Protein: BP000027-GD1 Syd Labs
