Recombinant Human SPARC Protein
Syd Labs Recombinant Human SPARC Protein BP000038-MPR11, Secreted Protein Acidic and Rich in Cysteine (SPARC, Basement-Membrane Protein 40, BM-40, Osteonectin, ON) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family.
- 产品参数
- 文献
- More Offers
| 货号 | BP000038-MPR11 |
|---|---|
| 产品名称 | Recombinant Human SPARC Protein |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Secreted Protein Acidic and Rich in Cysteine, Basement-Membrane Protein 40, BM-40, Osteonectin, ON |
| 来源 | Escherichia Coli-derived. |
| 形式 | Lyophilized from a sterile filtered solution of phosphate-buffered saline (PBS) |
| 纯度 | > 98% by SDS-PAGE and RP-HPLC analysis |
| 内毒素 | < 0.1 EU/ug SPARC as determined by the LAL method |
| 运输 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal SPARC Protein performance should be determined experimentally by the investigator. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000038-MPR11: Recombinant Human SPARC Protein
Background
Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 – 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.
BP000038-MPR11: Recombinant Human Secreted Protein Acidic and Rich in Cysteine (SPARC) Protein
Source: Escherichia Coli-derived.
Description: Glycosylated full-length (1-303) recombinant protein with a C-terminal His-6 tag.
Molecular weight: 34 kDa (predicted); 45 kDa (SDS-PAGE).
Purity: > 98% by SDS-PAGE and RP-HPLC analysis
Endotoxin: < 0.1 EU/ug SPARC as determined by the LAL method
Preservative: None
Sterility: 0.2 um membrane-filtered and packaged aseptically.
QC tests: SDS-PAGE, MALDI-TOF and RP-HPLC
Formulation: Lyophilized from a sterile filtered solution of phosphate-buffered saline (PBS)
Reconstitution: Add sterile distilled water to a concentration of no less than 0.1 ug/ml. Further dilutions should be made in appropriate buffers.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20°C to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20°C to -70°C under sterile conditions after reconstitution.
Please remember our product information: Recombinant Human SPARC Protein: BP000038-MPR11 Syd Labs