Recombinant Human PAK1 kinase domain Enzyme

Recombinant Human PAK1 kinase domain Enzyme: BP001573-K0104 Syd Labs

Recombinant Human PAK1 kinase domain Enzyme

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Syd Labs Recombinant Human PAK1 kinase domain Enzyme BP001573-K0104, Specific activity of Recombinant Human PAK1 Kinase Domain (PAK1KD) Enzyme: 32,638 pmoles/min/ug. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).

SKU: BP001573-K0104 分类: ,
货号 BP001573-K0104
产品名称Recombinant Human PAK1 kinase domain Enzyme
供货商名称 Syd Labs, Inc.
品牌名 悉得(Syd Labs)
别称 PAK1 Kinase Domain
来源 E. coli-derived.
形式 1 ug/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
运输 The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
注意事项 Conditions of optimal PAK1KD Enzyme performance should be determined experimentally by the investigator.
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描述

BP001573-K0104: Recombinant Human PAK1 kinase domain Enzyme

Background

Human PAK1 kinase (UniProt: Q13153; NCBI: NM_002576) belongs to a family of p21-activated kinases. These serine/threonine kinases serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 kinase regulates cell motility and morphology.

BP001573-K0104: Recombinant Human PAK1 Kinase Domain (PAK1KD) Enzyme

Source: E. coli-derived.
Predicted molecular mass: 33,646 Da. PAK1KD is fully phosphorylated at T423 and partially phosphorylated at additional sites with measured mass of 33,728 (1P), 33,808 (2P), 33,889 (3P), and 33,968 (4P).
Specific activity: 32,638 pmoles/min/?g. Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen).
Formulation: 1 ug/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.
Applications: Enzymatic studies, inhibitor screen, and selectivity profiling; High concentration available for crystallization.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.

Please remember our product information: Recombinant Human PAK1 kinase domain Enzyme: BP001573-K0104 Syd Labs

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