Recombinant Human FSH A/B Protein
Syd Labs Recombinant Human FSH A/B Protein BP000035-GD9, The activity of Follicle-Stimulating Hormone (FSH) α/β is measured in a human FSH receptor transfected HEK 293 cells. The ED50 for this effect is 0.1-0.4 ng/ml.
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| 货号 | BP000035-GD9 |
|---|---|
| 产品名称 | Recombinant Human FSH A/B Protein |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | 悉得（Syd Labs） |
| 别称 | Follicle-stimulating hormone |
| 来源 | Chinese Hamster Ovary cell line, CHO-derived. |
| 形式 | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4. |
| 纯度 | > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 内毒素 | <1.0 EU per 1 ug of the protein by the LAL method. |
| 运输 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| 注意事项 | Conditions of optimal FSH Protein performance should be determined experimentally by the investigator. |
| 线下下单 | 电话: 18162660842微信: duozhaozhao_2017Email: message@sydlabs.comOr 联系我们 |
描述
BP000035-GD9: Recombinant Human FSH A/B Protein
Background
Follicle-stimulating hormone (FSH) is a hormone found in humans and other animals. It is synthesized and secreted by gonadotrophs of the anterior pituitary gland. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. The protein dimer contains 2 polypeptide units (1)., labeled alpha and beta subunits. The alpha subunits contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHβ), which confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body.
BP000035-GD9: Recombinant Human FSH α/β Protein
Source: Chinese Hamster Ovary cell line, CHO-derived.
Human FSH α (Ala25 Ser116), Accession # P01215
Human FSH β (Cys21 Glu129), Accession # P01225
N-terminal sequence analysis: Ala25 (FSH α) & Cys21 (FSH β),
Predicted molecular mass: 10 kDa (FSH α) & 12 kDa (FSH β).
SDS-PAGE: About 25 kDa, reducing conditions.
The FSH α/β activity is measured in a human FSH receptor transfected HEK 293 cells. The ED50 for this effect is 0.1-0.4 ng/ml.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Reconstitution: Reconstitute at 100 ug/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Please remember our product information: Recombinant Human FSH A/B Protein: BP000035-GD9 Syd Labs