文献

重组抗小鼠CD3e单克隆抗体(克隆号145-2C11) ，人IgG1 Fc Silent Kappa（Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Human IgG1 Fc Silent Kappa，货号：PA007238.h1Fs Syd Labs）

145-2C11抗体可与成熟T细胞及髓质胸腺细胞特异性表达的T细胞受体-CD3复合体的CD3 epsilon（CD3ε）链相结合。

抗小鼠CD3e单抗(145-2C11)流式部分参考文献:

1. A novel trivalent non-Fc anti-CD3 Collabody preferentially induces Th1 cell apoptosis in vitro and long-lasting remission in recent-onset diabetic NOD mice
Huang CC, et al. Front Immunol. 2023 Aug 3;14:1201853. PMID: 37600814
“The V H and V L domains of the hamster anti-mouse CD3 ε antibody (clone 145-2C11) were grafted onto their respective human CH 1 and kappa light chain…Purified T cells were incubated with threefold serial dilutions of each anti-CD3 antibody ranging from 0 to 300 nM (145-2C11) or 600 nM (h145F(ab’) 2, h145chIgGAA, and h145CSA) for 1 h on ice, followed by staining with FITC-conjugated goat anti-hamster IgG (145-2C11) or Alexa Fluor 647-conjugated goat anti-human IgG (H+L)…”

2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Ocaña-Guzmán R, et al. Cells. 2022 May 13;11(10):1635. PMID: 35626672
“PE anti-mouse CD3 epsilon 145-2C11 100308…PE Armenian Hamster IgG isotype control…PE/Cy7 anti-mouse CD3 epsilon 145-2C11 100320…PE Armenian Hamster IgG isotype control HTK888 400922…Ultra-LEAF(TM) Purified anti-mouse CD3 antibody…”

3. A humanized CD3ε-knock-in mouse model for pre-clinical testing of anti-human CD3 therapy
Crespo J, et al. PLoS One. 2021 Feb 17;16(2):e0245917. PMID: 33596227
“Antibodies used for in vitro functional studies or in vivo treatment were anti-human CD3 (Clone OKT3), anti-mouse CD3 ε (Clone 145-2C11) and anti-mouse TCR β …Wells were coated overnight at 4°C with anti-human CD3 (OKT3, 1.0 μ g/mL), anti-mouse CD3 (145-2C11, 1.0 μ g/mL), or anti-mouse TCR β (1.0 μ g/mL), and their respective isotype controls…”

4. Entire CD3ε, δ, and γ humanized mouse to evaluate human CD3-mediated therapeutics
Ueda O, et al. Sci Rep. 2017 Apr 3;7:45839. PMID: 28368009
“For splenic T cell–stimulation via CD3, splenocyte cells were cultured on a plate coated with antibody to mouse Cd3 (145-2C11) or antibody to human CD3 (HIT3a)…Bispecific antibody against hGPC3 and mCD3 was generated by Fab arm exchange using anti-GPC3 antibody (GC33) and anti-mouse CD3 antibody (2C11)…Flow cytometric analysis demonstrated that human CD3+ cells were detected in splenocytes in three lines of human CD3 EDG–replaced mice…”

5. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sugita S, et al. Arthritis Res Ther. 2017 Jul 25;19(1):176. PMID: 28743289
“Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 μg/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min…Using flow cytometry, we first determined whether Dow2 can bind CD3 on mouse T cells by comparing its staining profile with two control anti-mouse CD3 Abs, 17A2 and 145-2C11…”

6. Differential depletion of total T cells and regulatory T cells and prolonged allotransplant survival in CD3ε humanized mice treated with polyclonal anti human thymocyte globulin
Buszko M, et al. PLoS One. 2017 Mar 1;12(3):e0173088. PMID: 28257450
“In murine research, a monoclonal anti-mouse CD3 ε mAb (clone 145-2C11) is used as a surrogate for OKT3 and it has been shown that in vivo administration of high doses of this mAb prolongs transplant survival…In contrast, low doses of 145-2C11 reversed spontaneous diabetes in NOD mice and decreased lipid accumulation in LDLr -/- mice, thus inhibiting atherosclerosis progression…”