文献

重组抗小鼠CD3单克隆抗体(克隆号17A2) ，人IgG1 Fc Silent Kappa（Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Human IgG1 Fc Silent Kappa，货号：PA007254.h1Fs Syd Labs）

抗小鼠CD3单抗（又称17A2抗体）是一种特异性识别小鼠T细胞受体（TCR）复合体中CD3ε链的单克隆抗体。CD3分子与TCR非共价结合，在T细胞抗原识别及信号转导过程中发挥核心调控作用，主要表达于成熟T细胞及胸腺细胞表面。在现代免疫学研究中，17A2抗体被广泛应用于流式细胞术中T细胞亚群的精准检测、免疫表型分析，以及在体内实验中用于T细胞激活、刺激或特定免疫调节干预。通过结合并交联TCR-CD3复合体，抗小鼠CD3单抗能够有效模拟抗原刺激，诱导T细胞增殖与活化，是研究细胞免疫应答机制、自身免疫性疾病模型及肿瘤免疫学不可或缺的重要工具。

作为专业的生命科学试剂供应商，Syd Labs推出的重组抗小鼠CD3单抗（克隆号17A2）采用高效的哺乳动物细胞表达系统进行重组生产，确保了产品高纯度与卓越的批次一致性。该产品属大鼠IgG2b Kappa同型格式，完全达到严格的体内实验级标准，具备超低的内毒素水平，能够有效避免非特异性刺激与细胞毒性干扰。无论是用于流式细胞术多色分析还是开展高要求的体内功能实验与免疫干预研究，Syd Labs重组17A2抗体都能为您提供高特异性与可靠的实验数据支持。

抗小鼠CD3单抗(17A2)流式部分参考文献:

1. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sugita S, Shimizu J, Makabe K, Keino H, et al. Arthritis Res Ther. 2017 Jul 25;19(1):176. PMID: 28743289
“In brief, CFSE-labeled C57BL/6JJcl splenocytes (2 × 10^6 cells/well) and irradiated (20 Gy) BALB/c splenocytes (2 × 10^5 cells/well) were cocultured in 24-well plates with 1 μg/ml of Dow2, a control anti-mouse CD3 ε Ab (clone 17A2), or an isotype control (rat IgG)…Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 μg/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min…”

2. In vivo Imaging Technologies to Monitor the Immune System
McCarthy CE, White JM, Viola NT, Gibson HM. Front Immunol. 2020 Jun 2;11:1067. PMID: 32582173
“Upon ex vivo analysis, one study reported a relative depletion of CD4 T cells and an expansion of CD8 T cells after delivery of an 89Zr-anti-CD3 tracer (clone 17A2) in mice…Conjugation of the contrast material to an antibody allows for specific targeting of a cell population or protein of interest…”

3. Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy
Larimer BM, Wehrenberg-Klee E, Caraballo A, Mahmood U. J Nucl Med. 2016 Oct;57(10):1607-1611. PMID: 27230929
“The monoclonal antimouse CD3 antibody clone 17A2 was purchased from…Conjugation and radiolabeling of the CD3 antibody resulted in a pure, highly specific probe for CD3-expressing cells…Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 10-μm slices of tumor to confirm the cellular location of CD3 expression…”