文献

重组抗小鼠CD11c单克隆抗体(克隆号N418) ，人IgG1 Fc Silent Kappa（Recombinant Anti-mouse CD11c Monoclonal Antibody (Clone: N418), Human IgG1 Fc Silent Kappa，货号：PA007485.h1Fs Syd Labs）

抗小鼠CD11c单抗（又称N418抗体）是一种特异性识别小鼠CD11c分子的单克隆抗体。CD11c亦被称为整合素alpha X（Integrin alpha X），是树突状细胞（Dendritic Cells, DCs）最经典且广泛使用的表面标志物之一，在髓系细胞的黏附、迁移及抗原提呈过程中发挥着重要作用。在现代免疫学与细胞生物学研究中，抗小鼠CD11c单抗（N418抗体）被广泛应用于流式细胞术中树突状细胞的鉴定与分群，以及在体内实验中用于研究抗原递呈机制、调节树突状细胞功能及探索免疫应答网络的调控机制。

作为专业的生命科学试剂供应商，Syd Labs推出的重组抗小鼠CD11c单抗（克隆号N418）采用高效的哺乳动物细胞表达系统进行重组生产，确保了批次间极高的稳定性与批次一致性。该产品完全达到严格的体内实验级标准，具备超低的内毒素水平，且采用Mouse IgG1 Kappa格式，能够满足高要求的动物活体实验与体外功能研究。无论是用于流式细胞术表型分析还是开展树突状细胞相关的体内免疫调节干预，Syd Labs重组N418抗体都能为您提供高特异性与可靠的实验数据支持。

抗小鼠CD11c单抗(N418)流式部分参考文献:

1. Rapid monoclonal antibody generation via dendritic cell targeting in vivo
Licea, et al. Hybrid Hybridomics. 2003 Feb;22(1):23-31. PMID: 12713687
“To address this question we immunized mice with IgG-peptide conjugates prepared with the hamster anti-murine CD11c MAb N418. …Synthetic peptides corresponding to two different exposed regions of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a human C-type lectin, were conjugated to N418 using thiol-based chemistry. …The N418 MAb served as the targeting molecule and synthetic peptides as the Ag (MAb-Ag). …A rapid and peptide specific serum IgG response was produced by Day 7 when the synthetic peptides were linked to the N418 MAb, compared to peptide co-delivered with the N418 without linkage.”

2. Rapid antibody responses by low-dose, single-step, dendritic cell-targeted immunization
Wang, H., et al. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):847-52. PMID: 10639168
“Although the exact cellular role of CD11c is unclear, binding of mAb N418 has been demonstrated not to interfere with the antigen presentation process of dendritic cells (15) and has been reported to induce an antibody response to itself after a single inoculation. …Thus, mAb N418 is an excellent candidate for antigen delivery. …The reporter protein antigen we chose is a polyclonal goat anti-hamster IgG antibody, which binds well to mAb N418 and is a foreign protein to the mouse…”