重组抗人HLA Class I Heavy Chain单抗(HC10)流式 | Syd Labs PA007483.m2a
重组抗人HLA Class I Heavy Chain单克隆抗体(克隆号HC10)，小鼠IgG2a Kappa(Syd Labs货号：PA007483.m2a)是用哺乳动物细胞生产的重组抗体，可用于流式细胞术和免疫组织化学-冷冻等研究，纯度>95%。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007483.m2a |
|---|---|
| 产品名称 | 重组抗人HLA Class I Heavy Chain单抗(HC10)流式 | Syd Labs PA007483.m2a |
| 英文名 | Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Mouse IgG2a Kappa (Clone: HC10) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA), HLA class I histocompatibility antigen |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | HC10 |
| 同种型 | 小鼠 IgG2a kappa |
| 特异性 | 该重组 HC10 抗体与人 HLA Class I Heavy Chain 蛋白特异性结合 |
| 免疫源 | 重组抗人HLA Class I Heavy Chain单克隆抗体 (克隆：HC10)在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，含0.09%叠氮化钠 |
| 偶联 | 非偶联 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 重组抗人HLA Class I Heavy Chain单克隆抗体，小鼠IgG2a Kappa(克隆号HC10)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 12个月，保存在2 至 8°C。 请勿冻结。 |
| 注意事项 | PA007483.m2a Syd Labs提供重组小鼠IgG2a同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | 流式细胞术（FC）和免疫组织化学-冷冻（IHC-F）。 |
文献
重组抗人HLA Class I Heavy Chain单克隆抗体(克隆号HC10)，小鼠IgG2a Kappa（Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Mouse IgG2a Kappa (Clone: HC10)，货号：PA007483.m2a Syd Labs）
人类主要组织相容性复合体I类分子（HLA Class I）重链是表达于绝大多数有核细胞表面的跨膜糖蛋白，在抗原递呈、T细胞识别以及NK细胞免疫监视中发挥核心调控作用。经典克隆号HC10抗体可特异性识别游离的HLA Class I重链（主要包括HLA-B和HLA-C重链，以及部分HLA-A重链结构），在分子未与β2-微球蛋白（β2m）结合或发生构象改变时仍能稳定结合。这一独特的构象特异性使其成为研究HLA分子合成折叠、内质网质量控制、HLA重链脱落以及肿瘤细胞免疫逃逸机制的重要工具，广泛应用于流式细胞术、免疫组化（IHC）、免疫沉淀（IP）及蛋白质印迹（Western Blot）等分析。
Syd Labs重组抗人HLA Class I Heavy Chain单抗(HC10)采用先进的哺乳动物表达系统重组合成，保证了批间高度一致性与出色的特异性。该产品采用体内实验级（In Vivo Grade）标准生产，遵循严格的低内毒素（Low Endotoxin）及无防腐剂控制，可有效避免体外细胞培养及体内模型实验中的细胞毒性与非特异性激活。配合Mouse IgG2a Kappa天然结构设计，在动物实验、免疫细胞功能阻断以及结构免疫学研究中表现出卓越的结合亲和力与稳定性，为肿瘤免疫和移植免疫领域的深度探索提供高质量且严谨可靠的试剂支持。
抗人HLA Class I Heavy Chain单抗(HC10)部分参考文献:
1. Endo-lysosomal assembly variations among human leukocyte antigen class I allotypes
Olson, E., et al. Elife. 2023;12:e79144. doi: 10.7554/eLife.79144
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) was used in peptide receptivity assays to measure specific expression and localization of HLA-B allotypes in donors selected from a genotyped cohort. Anti-Bw6 and anti-Bw4 antibodies, including HC10, facilitated analysis of homozygous or heterozygous HLA-B allotypes, ensuring minimal cross-reactivity with HLA-A or HLA-C allotypes.”
2. Non-conventional forms of HLA-B27 are expressed in spondyloarthritis joints and gut tissue
Rysnik, O., et al. J Autoimmun. 2016;70:12-21. doi: 10.1016/j.jaut.2016.03.011
“The HC10 antibody (anti-human HLA Class I Heavy Chain) and HD6 were used to detect non-conventional HLA-B27 (NC-B27) expression in human SpA joints, gut, and B27 TG 1 rat tissues via immunohistochemistry, flow cytometry, and confocal microscopy. High NC-B27 levels were observed in infiltrating and resident cells of inflamed joints and spleens of B27 TG 1 rats, increasing with age and disease progression.”
3. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats
Marroquin Belaunzaran, O., et al. PLoS One. 2015;10(6):e0130811. doi: 10.1371/journal.pone.0130811
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain, IgG2a) served as a positive control, binding β2m-free heavy chains of HLA-B and -C, including B272 and B27-β2m-free forms. Flow cytometry with HC10, HD6, and other antibodies (0.02 mg/mL) on LBL721.220 cells and leukocytes from transgenic rats confirmed cell-surface B272 expression, with secondary detection using PE-conjugated goat anti-mouse IgG.”
4. The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains
Jones, D. C., et al. PLoS One. 2015;10(6):e0129063. doi: 10.1371/journal.pone.0129063
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) blocked B27 dimer tetramer binding to LILRB5-transfected 293T cells, as confirmed by flow cytometry with W6/32, HC10, and ME1 antibodies. HC10 and anti-LILRB5 antisera inhibited B27 dimer binding, while multiparameter flow cytometry assessed LILRB5 surface expression on lymphocyte and monocyte populations.”
5. Psoriatic patients with arthropathy show significant expression of free HLA class I heavy chains on circulating monocytes: a potential role in the pathogenesis of psoriatic arthropathy
Lan, C. C., et al. Br J Dermatol. 2004;151(1):24-31. doi: 10.1111/j.1365-2133.2004.06016.x
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) detected free HLA class I heavy chains on circulating monocytes via flow cytometry in patients with psoriatic arthropathy. Peripheral blood mononuclear cells, isolated by Ficoll-Hypaque centrifugation, were stained with HC10 followed by FITC-conjugated goat anti-mouse IgG and analyzed using a FACScan flow cytometer with CellQuest software.”