NMab Protein G Resin

描述

IA123: NMab Protein G Resin

Background

Our recombinant protein G ligands are immobilized onto our proprietary agarose base matrices to provide cost-effective solutions for fast-processing of monoclonal and polyclonal antibodies, bispecific antibodies, and Fc fusion constructs with high quality.

* Rigid particles with outstanding pressure-flow properties; easy linear scale-up;
* Efficient mass transfer; high dynamic binding capacity; small elution volumes;
* Minimal non-specific binding; excellent antibody recovery;
* Animal component-free production; full technical and regulatory support.

IA123: NMab Protein G Resin

Our NMab protein G resin product range offers end users cost competitive and high perfomance Protein G affinity resins. NMab protein G resin is an agarose based Protein G resin developed using innovatively engineered protein G ligand and proprietary technologies of agarose crosslinking, surface extension, and ligand coupling. NMab protein G resin provides the combination of excellent binding capacity, low non-specific binding that leads to low impurity levels in the elution pool, good pressure-flow properties, and superior guanidine hydrochloride-tolerance for stability in the guanidine hydrochloride conditions used in Cleaning-in-place (CIP) and sanitization protocols. Table 1 lists the basic characteristics of this protein G resin:

Characteristics: Specification
Separation Function: Protein A affinity
Matrix: Cross-linked agarose
Ligand: recombinant protein G
Coupling Chemistry: Epoxy
Dynamic Binding Capacity: 55 – 65 mg/mL (human IgG, 5 minute residence time)
Maximum Pressure: 0.3 MPa
Clean in Place: 6 M guanidine hydrochloride
Recommended Flow Velocity: 100-500 cm/hour
pH Stability: 2-10
Chemical Stability: Stable in commonly used buffers, 20 mM sodium phosphate, 1% SDS, 6 M guanidine hydrochloride, 70% ethanol, 6M urea, etc. Avoid long-term exposure to strong acids or strong bases.
Operational Temperature: 2-40 ℃
Storage: 20% Ethanol or benzyl alcohol, 2-8 ℃

The engineered protein G in NMab protein G resin is manufactured with animal free raw materials.

NMab protein G resin provides reliable all around performance, low protein G leakage, high binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300 cm/hr). It is available in bottled (20% ethanol at 60% slurry concentration) or pre-packed column packaging.

For comparison, please see:

NMab Pro protein A resin
UniMab 50 HC Protein A resin

NMAB protein G resin, which combines the agarose bead and the recombinant protein G ligand using proprietary technologies of agarose crosslinking, surface extension, and ligand coupling, is the major component in pre-packed protein G columns.

Please remember our product information: NMab Protein G Resin: IA123 Syd Labs