Human B7-2/CD86 Protein C-His-Avi Tag | Syd Labs BP005656

Human B7-2/CD86 Protein BP005656 Syd Labs

Human B7-2/CD86 Protein C-His-Avi Tag | Syd Labs BP005656

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(悉得)Syd Labs 货号:BP005656 Human B7-2/CD86 Protein with the C-His-Avi Tag (人 B7-2/CD86 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.

货号 BP005656
产品名称Human B7-2/CD86 Protein C-His-Avi Tag | Syd Labs BP005656
供货商名称 Syd Labs, Inc.
品牌名 悉得(Syd Labs)
别称 CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2
物种 Human
来源 The recombinant Human B7-2/CD86 Protein, (amino acids Leu26-Pro247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Accession P42081-1
产品标签 C-His-Avi
生物素化 no
确定序列 Leu26-Pro247
分子量 The recombinant Human B7-2/CD86 protein with the His-Avi tag at the C-terminus has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
活性 Immobilized recombinant Human B7-2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CTLA-4 protein with the C-hFc tag with the EC50 of 0.12 ug/ml determined by ELISA.
Form Lyophilized
形式 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
制剂 It is recommended to reconstitute the lyophilized recombinant Human B7-2/CD86 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
纯度 >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
内毒素 Less than 1 EU per ug of protein as determined by LAL method.
运输 The recombinant Human B7-2/CD86 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
稳定性与储存 '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
注意事项 Conditions of optimal recombinant Human B7-2/CD86 Protein performance should be determined experimentally by the investigator.
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描述

BP005656: Human B7-2/CD86 Protein with the C-His-Avi Tag (人 B7-2/CD86 蛋白 C-His-Avi 标签)

 

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

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