Human B7-2/CD86 Protein C-His-Avi Tag | Syd Labs BP005656
(悉得)Syd Labs 货号:BP005656 Human B7-2/CD86 Protein with the C-His-Avi Tag (人 B7-2/CD86 蛋白 C-His-Avi 标签) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-Pro247.
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货号 | BP005656 |
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产品名称 | Human B7-2/CD86 Protein C-His-Avi Tag | Syd Labs BP005656 |
供货商名称 | Syd Labs, Inc. |
品牌名 | 悉得(Syd Labs) |
别称 | CD86 molecule; CD86; B70; B7-2 antigen; B72; B7-2; BU63; FUN-1; LAB72; MGC34413; CD28LG2 |
物种 | Human |
来源 | The recombinant Human B7-2/CD86 Protein, (amino acids Leu26-Pro247) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells. |
Accession | P42081-1 |
产品标签 | C-His-Avi |
生物素化 | no |
确定序列 | Leu26-Pro247 |
分子量 | The recombinant Human B7-2/CD86 protein with the His-Avi tag at the C-terminus has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
活性 | Immobilized recombinant Human B7-2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CTLA-4 protein with the C-hFc tag with the EC50 of 0.12 ug/ml determined by ELISA. |
Form | Lyophilized |
形式 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
制剂 | It is recommended to reconstitute the lyophilized recombinant Human B7-2/CD86 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening. |
纯度 | >95% by SEC-HPLC and SDS-PAGE under reducing conditions. |
内毒素 | Less than 1 EU per ug of protein as determined by LAL method. |
运输 | The recombinant Human B7-2/CD86 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
稳定性与储存 | '-20 to -80°C for 12 months as supplied from date of receipt.; -20 to -80°C for 3-6 months in unopened state after reconstitution.; 2-8°C for 2-7 days after reconstitution.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
注意事项 | Conditions of optimal recombinant Human B7-2/CD86 Protein performance should be determined experimentally by the investigator. |
线下下单 | 武汉多找找科技有限公司 电话: 18162581039 微信: duozhaozhao2024 Email: message@sydlabs.com或联系我们 |
描述
BP005656: Human B7-2/CD86 Protein with the C-His-Avi Tag (人 B7-2/CD86 蛋白 C-His-Avi 标签)
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
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