D-荧光素钾盐（D-Luciferin Potassium Salt）使用浓度是多少？

D-荧光素钾盐使用浓度是多少？从这些文献中找答案

D-荧光素钾盐（D-Luciferin Potassium Salt,CAS号:115144-35-9）使用浓度是多少？Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号MB000102-R70170或MB102-R70170），作为一项经过国际市场检验、主要用于动物体内实验的产品，在品质与成本效益上具有双重优势，这表明其在国际市场上具有较大的竞争力。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）现已强势登陆中国市场，期待为中国高校的动物实验中心和实验室注入新的活力。

Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt，货号：MB000102-R70170或MB102-R70170）因其卓越表现，在欧美多所知名大学的core facilities中获得高度认可，也成为了许多寻求高质量实验材料的研究人员的理想之选。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）的优质成效不仅体现在实际应用中，更在众多国际权威学术期刊中得以体现。Syd Labs D-荧光素钾盐（D-Luciferin Potassium Salt）被以下三篇文献引用：

48.

Visualizing cell–cell communication using synthetic notch activated MRI

TianDuo Wang,et al.

Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2216901120. doi: 10.1073/pnas.2216901120. Epub 2023 Mar 9.

For in vitro experiments, Jurkat T cell populations were co-cultured with CD19+ or CD19− Nalm6 cells. For a 1:1 ratio, 105 of each cell type was used. For varying target cell numbers, 105 T cells were used while the number of Nalm6 target cells was varied. For bioluminescence imaging (BLI) and flow cytometry, cells were mixed in a 96-well round bottom plate, whereas for microscopy, a 96-well flat bottom plate was used. After seeding the cell types in wells, plates were centrifuged at 400 g for 5 min to improve the interaction between cell types. For BLI, 150 μg/mL D-luciferin (Syd Labs) was added to each well and plates were immediately imaged on an IVIS Lumina XRMS scanner (PerkinElmer). Average radiance (p/s/cm2/sr) per well was quantified by placing regions-on-interest over each well using Living Image 4.5.2 software (PerkinElmer). To evaluate the percentage of tdT+ T cells, the percentage of BFP+ cells (from RE element) that were also tdT+ was evaluated via flow cytometry. To generate time-lapse movies, co-cultured cells were imaged using the CytoSMART Lux3 FL incubator microscope (CytoSMART Technologies BV). Brightfield (exposure: 9 ms) and tdT (exposure: 1,300ms, gain: 45, intensity: 90%) images were acquired immediately after mixing cells every 10 min for up to 40 h. Counts of tdT+ cells in microscopy images were obtained from CytoSMART software via the object count algorithm. To perform the OATP1B3 uptake assay, co-cultured T cells were collected at 24 h post-seeding and incubated with Live/Dead Fixable Green (LDG) Dye (ThermoFisher) according to the manufacturer’s instructions. Cells were then co-incubated with the Sytox Red Dead Cell Stain (ThermoFisher) to stain any dead cells. The percentage of live BFP+ cells that were also LDG+ was analyzed using flow cytometry. To evaluate toxicities from our gadolinium agent, at 24 h post-seeding, an appropriate volume of Gd-EOB-DTPA (Primovist; Bayer) was added to CD19+ co-cultures to achieve the indicated concentration in media. Cells were incubated with agent for 90 min, then washed and collected, and the percentage of live BFP+ cells (Sytox Red−) was analyzed using flow cytometry.

49.

Engineering “self-homing” circulating tumour cells as novel cancer theranostics

Katie M Parkins,et al.

Theranostics. 2020 Jun 29;10(17):7925-7937. doi: 10.7150/thno.44259. eCollection 2020.

All in vitro results are from three independent experiments with three replicates of each condition. To evaluate the relationship between cell number and BLI signal, 1×104, 5×104, 1×105, 1.5×105, and 5×105 4T1BR5-FLuc or 4T1-RLuc cells were seeded in each well of 24-well plates. We acquired fluorescent images of each plate. We then added 10 μL of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA) or 10 μL of h-Coelenterazine (150μg/mL; NanoLight Technology, Prolume, AZ, USA) to the growth medium in each well and BLI images were collected for up to 35 minutes. All images were acquired using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). Signal was measured with region-of-interest (ROI) analysis using LivingImage Software (Perkin Elmer). An ROI was drawn around each well to measure the radiant efficiency (p/s/cm2/sr/uW/cm2) for fluorescence images and average radiance (p/s/mm2/sr) for bioluminescence images. The mean signal across replicates was determined for each independent experiment.