Syd Labs D-Luciferin K Salt科学应用文献参考

Syd Labs D-Luciferin K Salt科学应用文献参考

D-Luciferin Potassium Salt(别名:D-Luciferin K Salt)是荧光素酶 (Luc) 的底物,能够催化生物发光昆虫产生典型的黄绿光。Syd Labs D-Luciferin K Salt MB000102-R70170可以用于荧光素酶实验。Syd Labs D-荧(萤)光素钾盐纯度可以达到99%以上,比国内市面上很多产品纯度要高,有效帮助科研工作者提高实验效果,但是价格却比国内的同类产品低!这也是为什么Syd Labs D-Luciferin K Salt能受到客户的青睐的主要原因,下面我们看看我们的国内外客户发表的与D-Luciferin应用相关的文献(其中应用到了Syd Labs D-Luciferin K Salt):

5.

Multimodality cellular and molecular imaging of concomitant tumour enhancement in a syngeneic mouse model of breast cancer metastasis

Katie M Parkins, Veronica P Dubois, Amanda M Hamilton, Ashley V Makela, John A Ronald, Paula J Foster

Sci Rep. 2018 Jun 12;8(1):8930. doi: 10.1038/s41598-018-27208-4.

All in vitro results are from three independent experiments with three replicates of each condition. To evaluate the relationship between cell number and BLI signal, 1 × 103, 5 × 103, 1 × 104, 1.5 × 105, and 5 × 105 4T1BR5-Red-FLuc/GFP cells were seeded in each well of 24-well plates. Twenty-four hours later, 5 μL of D-luciferin (30 mg/mL; Syd Labs, Inc., MA, USA) was added to the growth medium and BLI images were collected 5 minutes later using a hybrid optical/X-ray scanner (IVIS Lumina XRMS In Vivo Imaging System, PerkinElmer). BLI signal was measured with region-of-interest (ROI) analysis using LivingImage Software (Perkin Elmer). An ROI was drawn around each well to measure the average radiance (photons/second/mm2/steradian), and the mean average radiance across replicates was determined for each independent experiment.

6.

Effect of Cholesterol Content of Lipid Composition in mRNA-LNPs on the Protein Expression in the Injected Site and Liver After Local Administration in Mice

Maho Kawaguchi, Marin Noda, Akari Ono, Mariko Kamiya, Makoto Matsumoto, Masako Tsurumaru, Shusaku Mizukami, Hidefumi Mukai, Shigeru Kawakami

J Pharm Sci. 2023 May;112(5):1401-1410. doi: 10.1016/j.xphs.2022.12.026. Epub 2022 Dec 31.

For in vivo imaging system (IVIS) imaging evaluation, we selected luciferase mRNA SS-OP LNPs containing 10, 20, and 40 mol% cholesterol. Naked FLuc mRNA or FLuc mRNA-LNP was administered intramuscularly at a dose of 2 µg mRNA per mouse. One hundred microliters of 30 mg/mL D-luciferin (Syd Labs, Inc., Natick, MA) solution was injected intraperitoneally at 4.5, 9, 24, 48, and 72 h after mRNA-LNP administration, and luciferase protein expression was observed by IVIS imaging (IVIS Lumina II, Caliper Life Sciences, MA) under 2% isoflurane anesthesia. The value of the total flux in the muscle to that in the liver was calculated from the imaging results. It is included as a reference value to characterize the luciferase expression among muscle and liver.

Syd Labs D-Luciferin K Salt

Syd Labs公司Syd Labs D-Luciferin K Salt MB102产品覆盖各大学科研所和医院里的动物研究以及生物医学研究机构的科学家,已经获得广大科研工作者认可。D-荧光素钾盐(D-Luciferin K)在进口D-荧光素钾盐中价格最低。

武汉多找找科技是Syd Labs中国区合作伙伴,为科研工作者提供优质的产品与服务。Syd Labs D-Luciferin K Salt MB000102-R70170 或 MB102 D-荧光素钾盐价格或其它更多产品参数信息,来自于武汉多找找科技,欢迎大家询单。

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