抗小鼠CTLA-4单克隆抗体(克隆号9D9) ，体内实验级重组，小鼠IgG2c-LALAPG Kappa | Syd Labs PA007380.m2cLA
体内实验级重组抗小鼠CTLA-4单克隆抗体，小鼠IgG2c-LALAPG Kappa (克隆号：9D9，货号：PA007380.m2cLA）是用哺乳动物细胞生产的重组抗体，可用于ELISA、流式细胞术、中和、功能测定，如生物分析PK和ADA测定，以及研究受小鼠CTLA-4蛋白影响的生物途径的测定，比如体内CTLA-4中和、肿瘤内调节性T细胞耗竭。Syd Labs PA007380.m2cLA不变区为小鼠IgG2c-LALAPG Kappa，可与重组小鼠IgG2c-LALAPG同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
应用指南：9D9抗体在不同小鼠肿瘤模型中剂量建议
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| 货号 | PA007380.m2cLA |
|---|---|
| 产品名称 | 抗小鼠CTLA-4单克隆抗体(克隆号9D9) ，体内实验级重组，小鼠IgG2c-LALAPG Kappa | Syd Labs PA007380.m2cLA |
| 英文名 | In vivo Grade Recombinant Anti-mouse CTLA-4 Monoclonal Antibody, Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa (Clone: 9D9) |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 细胞毒性T淋巴细胞相关抗原4，细胞毒性T细胞抗原4，CD152，CTLA4 |
| 概述 | Syd Labs提供重组小鼠IgG2c-LALAPG同型对照抗体，样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | 9D9 |
| 同种型 | 小鼠 IgG2c, kappa |
| 应用 | ELISA、流式细胞术（flow cytometry）、中和（neutralization）、功能测定（functional assays），如生物分析PK和ADA测定，以及用于研究受小鼠CTLA-4蛋白影响的生物途径的测定。 |
| 免疫源 | 抗小鼠CTLA-4单克隆抗体（克隆：9D9）在哺乳动物细胞中产生。 |
| 抗体形式 | 0.2 μM过滤溶液，pH 7.4，不含稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗小鼠CTLA-4单克隆抗体，小鼠IgG2c-LALAPG Kappa(克隆号9D9)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C，自收到之日起可保存1个月。 如果保存在-20 至 -70°C，自收到之日起可保存 12个月。 |
| 注意事项 | PA007380.m2cLA Syd Labs提供重组小鼠IgG2c-LALAPG同型对照抗体，样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
描述
PA007380.m2cLA: Syd Labs体内实验级重组抗小鼠CTLA-4单克隆抗体（克隆号9D9），小鼠IgG2c-L234A L235A P329G (LALAPG) Kappa（In vivo Grade Recombinant Anti-mouse CTLA-4 Monoclonal Antibody, Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa (Clone: 9D9)）
抗小鼠CTLA-4抗体（9D9）部分参考文献:
Induced PD-L1 expression mediates acquired resistance to agonistic anti-CD40 treatment.
Zippelius, A., et al. Cancer Immunol Res. 2015 Mar;3(3):236-44. doi: 10.1158/2326-6066.CIR-14-0226. PMID: 25623164
Concomitant CTLA-4 blockade further improved the therapeutic efficacy of this combination. …Owing to their distinct immunologic mechanisms of action, concomitant blockade of PD-1 and CTLA-4 has shown superior therapeutic activity in patients with melanoma as compared with either of the antibodies alone. …Intriguingly, we have noticed an increase in the frequency of CTLA-4–expressing CD8+ T cells (Supplementary Fig. S1F and S1G) upon agonistic anti-CD40 treatment. …When combining both anti–PD-L1 and anti–CTLA-4 with anti-CD40 treatment, more than 90% of tumors were rejected. …By integrating CTLA-4–blocking antibodies into the anti-CD40/PD-L1 and anti-CD40/PD-1 treatment regimen, we were able to further enhance durable tumor rejection. …Although a clinical trial evaluating the agonistic anti-CD40 antibody RO7009789 in combination with the CTLA-4–blocking antibody tremelimumab is currently under way at the University of Pennsylvania, to our knowledge, a combination with PD-1/PD-L1–blocking antibodies has not yet been attempted in patients with cancer.
Tags: anti-mouse CTLA-4 9D9; anti-mouse CTLA-4 9D9 mAb
Combined targeting of costimulatory (OX40) and coinhibitory (CTLA-4) pathways elicits potent effector T cells capable of driving robust antitumor immunity.
Redmond, W. L., et al. Cancer Immunol Res. 2014 Feb;2(2):142-53. doi: 10.1158/2326-6066.CIR-13-0031-T. PMID: 24778278
CTLA-4 is a negative regulatory protein that serves as a checkpoint inhibitor to limit T-cell responses by attenuating T-cell proliferation and cytokine production. …OX40 and CTLA-4 are both upregulated on CD4 and CD8 T cells shortly after TCR stimulation and are constitutively expressed on Tregs. …Preclinical data have shown that monotherapy with anti-OX40 or anti-CTLA-4 has limited therapeutic efficacy against many tumor types, suggesting that combination immunotherapy likely will be required to generate optimal therapeutic responses. …These data provide insight into the mechanisms by which the combined anti-OX40/anti-CTLA-4 immunotherapy drives potent polyclonal effector T-cell (Teff) responses and suggest that the clinical combination of these agents may offer a novel means of boosting tumor immunotherapy in patients with cancer. …Monotherapy with anti-OX40 or anti-CTLA-4 had limited effects on tumor growth or survival, but the combined immunotherapy significantly enhanced tumor regression and survival.
Tags: anti-mouse CTLA-4 9D9 in vivo; anti-mouse CTLA-4 9D9 in animal model
Combinatorial PD-1 blockade and CD137 activation has therapeutic efficacy in murine cancer models and synergizes with cisplatin.
Wei, H., et al. PLoS One. 2013 Dec 19;8(12):e84927. doi: 10.1371/journal.pone.0084927. PMID: 24367702
Programmed Death 1 (PD-1) protein is a co-inhibitory receptor on T cells with a structure similar to that of CTLA-4 but with a distinct biologic function and ligand specificity. …Based on published evidence indicating a therapeutic potentials in several tumor models we tested mAbs to CD137, CD40, CTLA-4, PD-1, TIM-3 and LAG-3 [34,35], as single agents and in combinations. …The following monoclonal antibodies (mAb) used in animal experiments were purchased from BioXcell (West Lebanon, NH): anti-CD137 (Clone lob12.3), anti-PD-1 (Clone RMP1-14), anti-CTLA4 (Clone 9D9), anti-NK1.1 (Clone PK136), anti-CD8 (Clone 2.43), anti-CD4 (Clone GK1.5) and control (Clone 2A3). …Mice were injected intraperitoneally (i.p.) with 3 × 106 ID8 cells in 0.1 mL of PBS. On days 10 and 14 after tumor inoculation, mice were injected i.p. with 0.5 mg of each mAb in total 0.5 mL of PBS as shown in the figure legends. …Mice which had been transplanted i.p. with ID8 cells were euthanized 7 and 14 days after they had been injected with the anti-PD1, anti-CD137, anti-PD1/CD137 or control as in the therapy experiments.
Tags: anti-mouse CTLA-4 9D9 mAb in animal model; function of anti-mouse CTLA-4 9D9
了解更多抗小鼠CTLA-4单克隆抗体（clone：9D9）参考文献，请查看：抗小鼠CTLA-4抗体（克隆号9D9）参考文献
Syd Labs抗小鼠CTLA-4重组抗体（克隆号9D9），小鼠IgG2c-LALAPG Kappa（货号：PA007380.m2cLA）推荐同型对照抗体:
Syd Labs相关重组IgG参考抗体（Related Recombinant IgG Reference Antibodies）:
重组小鼠IgG1同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants）
重组小鼠IgG2a同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants）
重组小鼠IgG2c同型对照抗体和突变体，体内实验级（In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants）
Syd Labs提供以下研究级抗CTLA-4抗体生物类似药（research grade anti-CTLA-4 antibody biosimilars）:
Ipilimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Tremelimumab Biosimilar, research grade, anti-human CTLA-4 monoclonal antibody
Syd Labs提供以下重组CTLA-4蛋白（Recombinant CTLA-4 Proteins）:
Biotinylated Human CTLA-4 Protein
Cynomolgus CTLA-4 Protein
Human CTLA-4 Protein
Syd Labs还提供以下体内实验级重组抗小鼠CTLA-4单克隆抗体(克隆号9D9)及其突变体:
体内实验级重组抗小鼠CTLA-4单克隆抗体，小鼠IgG2b Kappa(克隆号9D9)
体内实验级重组抗小鼠CTLA-4单克隆抗体，小鼠IgG2a-LALAPG Kappa(克隆号9D9)
背景知识
The 9D9 antibody（9D9抗体、抗小鼠CTLA-4单抗9D9） blocks the mouse cytotoxic T lymphocyte antigen-4 (CTLA-4). Blocking CTLA-4 removes an inhibitory signal from reducing the activity of T lymphocytes.
Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an inhibitory molecule that competes with the stimulatory CD28 for binding to B7 on antigen presenting cells. CTLA-4 and CD28 are both presented on the surface of T-cells.
请记住我们的产品信息: 体内实验级重组抗小鼠CTLA-4单克隆抗体，小鼠IgG2c-LALAPG Kappa(克隆号9D9): PA007380.m2cLA Syd Labs In vivo Grade Recombinant Anti-mouse CTLA-4 Monoclonal Antibody, Mouse IgG2c-L234A L235A P329G (LALAPG) Kappa (Clone: 9D9)。