文献

重组抗小鼠CD40单克隆抗体(克隆号FGK4.5) ，大鼠IgG2a Kappa（Recombinant Anti-mouse CD40 Monoclonal Antibody (Clone: FGK4.5), Rat IgG2a Kappa，货号：PA007315.r2a Syd Labs）

重组FGK4.5抗体可与小鼠CD40蛋白结合。CD40是一种I型跨膜蛋白，表达于抗原呈递细胞表面，对其活化至关重要。辅助性T细胞（Th细胞）表面的CD40L/CD154与CD40的结合能够激活抗原呈递细胞并诱导一系列下游效应。活化的CD4+ T细胞主要表达其配体CD40L/CD154，该配体可与多种细胞表面的CD40结合，这些细胞包括树突状细胞（DCs）、B细胞、巨噬细胞、经典及非经典单核细胞等抗原呈递细胞，血小板和内皮细胞等多种非免疫细胞，以及多种类型的肿瘤细胞。

抗小鼠CD40单抗(FGK4.5)流式部分参考文献:

1. CD40 agonistic antibody triggers an immature DC signature in breast cancer
Mokhtari, R. B., et al. PLoS One. 2014 Apr 16;9(4):e94515. PMID: 24740297
“Splenocytes were stimulated with LPS (1 μg/ml) or anti-mouse CD40 antibody (FGK4.5 clone, 10 μg/ml) for 24 h to induce DC maturation. Flow cytometry analysis showed increased CD80 and CD86 expression on CD11c+ DCs after FGK4.5 stimulation. The FGK4.5 antibody induced an immature DC phenotype in tumor-bearing mice, with reduced MHC-II levels…”

2. Agonistic anti-CD40 antibody-driven IL12: a novel strategy to boost DC vaccine potency
Kim, H. M., et al. J Immunol. 2012 Dec 15;189(12):6102-10. PMID: 23175440
“Anti-mouse CD40 mAb (clone FGK4.5) was injected intraperitoneally at 100 μg/mouse to activate DCs in vivo. Flow cytometric analysis of splenocytes demonstrated enhanced CD86 and MHC class II expression on DCs after FGK4.5 administration. IL-12 production was upregulated in FGK4.5-treated DCs, as measured by intracellular staining…”

3. CD40 signaling synergizes with TLR signaling in DCs to induce potent T cell immunity
Ahonen, C. L., et al. J Immunol. 2010 May 1;184(9):5039-49. PMID: 20363975
“Bone marrow-derived DCs were matured with LPS plus anti-mouse CD40 (FGK4.5, 5 μg/ml) for flow cytometry phenotyping. FGK4.5 stimulation synergized with TLR ligands to upregulate costimulatory molecules on DCs, confirmed by flow cytometry. Adoptive transfer of FGK4.5-matured DCs into OT-I mice enhanced antigen-specific CD8+ T cell proliferation…”

4. Therapeutic antitumor response after anti-CD40 immunotherapy in B lymphoma
Houot, R., et al. Blood. 2005 Dec 1;106(12):4089-96. PMID: 16210641
“Mice were treated with anti-mouse CD40 mAb (FGK4.5, 100 μg i.p.) to activate APCs in the A20 lymphoma model. Flow cytometry revealed transient B cell activation with increased CD86 expression post-FGK4.5 injection. The FGK4.5 antibody induced tumor regression in 60% of treated mice…”

5. In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity
Grewal, I. S., et al. J Immunol. 1997 Jan 15;158(2):721-30. PMID: 9006927
“Anti-mouse CD40 antibody (FGK4.5 clone) was used to block CD40 signaling in vivo during T-dependent responses. Flow cytometric analysis of splenocytes showed impaired germinal center B cell formation after FGK4.5 blockade. Immunoglobulin isotype switching was reduced in FGK4.5-treated mice immunized with TNP-KLH…”