重组抗小鼠CD25单抗(PC61.5.3) | Syd Labs PA007156.mf
由 CHO 或工程化 CHO 细胞株表达生产的重组抗小鼠 CD25 小鼠 IgG2c 单抗（克隆号：PC61、PC61.5 或 PC61.5.3），适用于体外和体内研究。其中，无岩藻糖化（afucosylated）抗体可能在体内调节性 T 细胞（Treg）耗竭/清除中表现更佳。
产品参数
| 货号 | PA007156.mf |
|---|---|
| 产品名称 | 重组抗小鼠CD25单抗(PC61.5.3) | Syd Labs PA007156.mf |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 克隆号 | PC61, PC61.5 or PC61.5.3. |
| 应用 | immunoprecipitation (IP), immunohistochemistry (IHC), Flow Cytometry (FC), and various functional assays, such as in vivo depletion of CD25+CD4+ Treg cells, in vitro blocking of IL-2 binding to low- and high-affinity receptors, and growth inhibition of IL-2-dependent T-cell lines. |
| 免疫源 | IL-2-dependent cytolytic mouse T-cell clone B6.1. |
| 抗体形式 | 0.2 μM filtered solution of 1x PBS. |
| 内毒素 | Less than 1 EU/mg of protein as determined by LAL method. |
| 纯度 | >95% by SDS-PAGE under reducing conditions. |
| 运输 | The in vivo grade recombinant anti-mouse CD25 mouse IgG2c kappa monoclonal antibodies are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| 稳定性与存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied. |
| 注意事项 | Recombinant anti-mouse CD25 mouse IgG2c monoclonal antibodies (clone: PC61, PC61.5 or PC61.5.3) produced from CHO or engineered CHO cell lines good for in vitro and in vivo studies. The afucosylated antibody may behave better in in vivo regulatory T cell depletion. |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
文献
PA007156.mf: 重组抗小鼠CD25单克隆抗体(克隆号：PC61.5.3)，小鼠 Fab Fragment，体内实验级（In vivo Grade Recombinant Anti-mouse CD25 (Clone PC61.5.3) Mouse Fab Fragment ）
体内实验级重组抗小鼠 CD25 单克隆抗体基于克隆号为 PC61、PC61.5 或 PC61.5.3 的大鼠抗小鼠 CD25 单克隆抗体。该大鼠 IgG1 Lambda 单克隆抗体已被验证并报道可用于免疫沉淀（IP）、免疫组化（IHC）、流式细胞术（FC）以及多种功能分析实验，例如体内 CD25+CD4+ Treg 细胞的耗竭/清除、体外阻断 IL-2 与低亲和力及高亲和力受体的结合，以及抑制 IL-2 依赖性 T 细胞株的生长。
CD25 与 CD4 及 FOXP3 的共同表达被视为 Treg 细胞的特征性表型。使用大鼠 PC61 单克隆抗体对小鼠 CD25(+)CD4(+)FoxP3(+) Treg 细胞进行体内耗竭，已被广泛应用于解析和表征 Treg 细胞的功能。PC61 单克隆抗体特异性结合 CD25 上一个独特的抗原表位，该表位不同于 IL-2 结合位点，也不同于 3C7 和 7D4 单克隆抗体所识别的表位。通过这种特异性结合，该抗体能够有效阻断 IL-2 与 CD25 的结合。
抗小鼠 CD25 单克隆抗体（克隆号：PC61.5.3）参考文献：
Soler-Cardona, et al. Cancers (Basel). 2021 Nov 16;13(22):5730. PMID: 34827083
“Targeting CD25 (IL-2Rα) to deplete regulatory T cells (Tregs) is a promising strategy to enhance antitumor immunity. … We generated and evaluated two novel human anti-CD25 monoclonal antibodies with distinct binding characteristics. … Interestingly, our results revealed that the antitumor efficacy in syngeneic models was inversely related to antibody affinity and in vitro neutralizing efficacy.”
2. TRAIL suppresses tumor growth in mice by inducing tumor-infiltrating CD4+CD25+ Treg apoptosis.
Wang, et al. Cell Death Dis. 2024 Apr 19;15(4):276. PMID: 38641998
“Tumor-infiltrating CD4+CD25+ regulatory T cells (Tregs) heavily contribute to the immunosuppressive tumor microenvironment. … This study evaluates how the TRAIL pathway impacts the survival and frequency of these regulatory populations in mouse tumor models. … The findings indicate that TRAIL delivery effectively suppresses overall tumor growth by selectively inducing apoptosis in CD4+CD25+ Tregs.”
3. A distinct subpopulation of CD25− T-follicular regulatory cells localizes in the germinal centers.
Wing, et al. Proc Natl Acad Sci U S A. 2017 Aug 1;114(31):E6400-E6409. PMID: 28716922
“T-follicular regulatory (Tfr) cells act to control the magnitude of the germinal center B cell response to maintain self-tolerance. … Here, we identify and characterize a prominent subpopulation of Tfr cells that lack CD25 expression. … These unique CD25− Tfr cells selectively localize inside germinal centers and display potent suppressive functions compared to classical CD25+ populations.”
4. IL-2 can signal via chemokine receptors to promote regulatory T cells’ suppressive function.
Luo, et al. Nat Commun. 2023 Oct 10;14(1):6332. PMID: 37816739
“Interleukin-2 (IL-2) is heavily recognized as a core driver of regulatory T cell (Treg) activation and survival via the high-affinity CD25 receptor. … We discovered an alternative mechanism where IL-2 directly interacts with chemokine receptors on T cells. … This alternative signaling axis enhances Treg suppressive activity and migration independently of conventional CD25-mediated signaling pathways.”
5. Mast cells suppress murine GVHD in a mechanism independent of CD4+CD25+ regulatory T cells.
Leveson-Gower, et al. Blood. 2013 Nov 21;122(22):3659-3667. PMID: 24092937
“Graft-versus-host disease (GVHD) is a major life-threatening complication associated with allogeneic hematopoietic cell transplantation. … Although CD4+CD25+ regulatory T cells are vital for curbing GVHD severity, other suppressive pathways exist. … Our study shows that donor-derived mast cells significantly ameliorate GVHD symptoms via an independent mechanism that does not require host or donor CD4+CD25+ Tregs.”
您可能还会喜欢…
-
阅读更多Quick View