重组抗人CD56单抗(N901) | Syd Labs PA007278.m1
Syd Labs体内实验级重组抗人CD56单克隆抗体(克隆号N901)，小鼠IgG1 Kappa（货号：PA007278.m1）是用哺乳动物细胞生产的重组抗体，适用于体外和体内研究，纯度>95%。Syd Labs PA007278.m1不变区为小鼠Mouse IgG1 Kappa (mIgG1或m1)，可与重组小鼠IgG1同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007278.m1 |
|---|---|
| 产品名称 | 重组抗人CD56单抗(N901) | Syd Labs PA007278.m1 |
| 英文名 | In Vivo Grade Recombinant Anti-human CD56 Monoclonal Antibody（Clone N901）,Mouse IgG1 Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 神经细胞粘附分子，ncAM，CD56，MSk39，神经元黏附分子，ncAM1 |
| 概述 | Syd Labs提供重组小鼠 IgG1同型对照抗体和重组人 IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | N901 |
| 同种型 | 小鼠 IgG1 Kappa |
| 免疫源 | 抗人 CD56单克隆抗体(克隆号: N901)是从哺乳动物细胞中生产的 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人CD56单克隆抗体(克隆号N901)，小鼠IgG1 Kappa用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 如果保存在2 至 8°C，自收到之日起可保存3个月。如果保存在-20 至 -70°C，自收到之日起可保存 12个月。 |
| 注意事项 | PA007278.m1 Syd Labs提供重组小鼠 IgG1同型对照抗体和重组人 IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受人 CD56蛋白影响的生物学途径的测定。 |
文献
PA007278.m1: Syd Labs 抗人CD56单克隆抗体(克隆号N901) ，体内实验级重组，小鼠IgG1 Kappa（In vivo Grade Recombinant Anti-human CD56 Monoclonal Antibody (Clone: N901), Mouse IgG1 Kappa）
重组N901抗体（抗人CD56单克隆抗体N901）与人CD56蛋白结合。CD56是一种同源结合糖蛋白，表达于神经元、胶质细胞和骨骼肌表面。CD56是神经谱系定向的标志物。CD56表达于造血系统、自然杀伤细胞以及其他淋巴样细胞（包括γδ T细胞和活化的CD8+ T细胞），也表达于树突状细胞。CD56在细胞间黏附、神经突生长、突触可塑性以及学习与记忆中发挥作用。
抗人CD56单抗(N901)部分引用文献:
1. Advanced Immune Cell Profiling by Multiparameter Flow Cytometry in Humanized Patient-Derived Tumor Mice
Bruss, C., et al. Cancers (Basel). 2022 Apr 28;14(9):2214. PMID: 35565343
“The N901 antibody (anti-human CD56, PE-conjugated) was used in multiparameter flow cytometry to characterize immune cells in humanized patient-derived tumor mice. …Staining was performed on spleen single-cell suspensions and peripheral blood using FACSCanto-II or Navios cytometers, with DuraClone protocols, to assess CD56 expression alongside markers like CD3, CD19, and CD45 for immune profiling.”
2. Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications
Blache, U., et al. Front Immunol. 2021 May 3;12:658314. PMID: 34012442
“The N901 antibody (anti-human CD56) was included in antibody panels (TCR, RE ALB, CAR-T1, CAR-T2, NK1, NK2) for staining EDTA blood samples in CAR-T cell immune monitoring. …Flow cytometry using a DxFLEX cytometer analyzed NK cell populations, with 100 µl whole blood stained for 15 minutes to assess CD56 expression in CAR-T therapy contexts.”
3. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes
van Dongen, J. J. M., et al. Leukemia. 2012 Sep;26(9):1908-39. PMID: 22552007
“The N901 antibody (anti-human CD56) was part of the EuroFlow antibody panels for standardized flow cytometric immunophenotyping of leukocytes. …Used with EuroFlow SOPs on FACSCanto-II cytometers and Infinicyt software, it facilitated consistent analysis of CD56 expression in normal, reactive, and malignant leukocytes, supporting WHO-based diagnosis of hematological malignancies.”
4. Detection of the CD56+/CD45- immunophenotype by flow cytometry in neuroendocrine malignancies
Bryson, G. J., et al. J Clin Pathol. 2002 Jul;55(7):535-7. PMID: 12101203
“The N901 antibody (anti-human CD56, PE-conjugated) was used in a three-color flow cytometry assay (with anti-CD3-FITC and anti-CD45-PECY5) on a Beckman Coulter XL cytometer to identify the CD56+/CD45- immunophenotype in neuroendocrine malignancies, such as metastatic small cell carcinoma, confirming its utility in lymphoma evaluation.”
5. Aggressive natural killer cell leukemia/lymphoma with N901-positive surface phenotype: evidence for the existence of a third lineage in lymphoid cells
Imamura, N., et al. Acta Haematol. 1988;80(3):157-64. PMID: 2461636
“The N901 antibody (anti-human CD56, NKH-1) identified CD56 expression on leukemic cells with high NK activity in aggressive NK cell leukemia/lymphoma. …Flow cytometry showed these cells lacked Leu 7 (HNK-1) and T/B-cell lineage markers, confirming a distinct third lymphoid lineage without T beta, T gamma, or immunoglobulin gene rearrangement.”