重组抗人CD3e单抗(12F6) | Syd Labs PA007387.m2a
体内实验级重组抗人CD3e单克隆抗体，小鼠IgG2a Kappa（克隆号：12F6，Syd Labs货号：PA007387.m2a），纯度>95%，适用于体外和体内研究。其不变区为小鼠Mouse IgG2a Kappa (mIgG2a或m2a)，可与重组小鼠IgG2a同型对照抗体配套使用。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。
产品参数
| 货号 | PA007387.m2a |
|---|---|
| 产品名称 | 重组抗人CD3e单抗(12F6) | Syd Labs PA007387.m2a |
| 英文名 | In Vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa |
| 供货商名称 | Syd Labs, Inc. |
| 品牌名 | Syd Labs |
| 别称 | 分化簇 3，CD3D，CD3E，CD3G |
| 概述 | Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 克隆号 | 12F6 |
| 同种型 | 小鼠 IgG2a kappa |
| 免疫源 | 抗人CD3e单克隆抗体（克隆：12F6）在哺乳动物细胞中产生 |
| 抗体形式 | 0.2微米过滤溶液，pH 7.4，无稳定剂或防腐剂 |
| 内毒素 | 根据 LAL 方法，≤1 EU每1mg 蛋白质 |
| 纯度 | >95%（在还原条件下通过SDS-PAGE测定） |
| 运输 | 体内实验级重组抗人CD3e单克隆抗体，小鼠 IgG2a Kappa(克隆号12F6)用冰袋运输。收到后，请立即将其存放在下面建议的温度下。 |
| 稳定性与存储 | 使用手动除霜冰箱并避免重复冻融循环。 自收到之日起 1 个月，保存在2 至 8°C。 自收到之日起12个月，保存在-20 至 -70°C。 |
| 注意事项 | PA007387.m2a Syd Labs提供重组小鼠IgG2a同型对照抗体和重组人IgG1同型对照抗体。样品制备条件和最佳样品稀释度应由研究人员通过实验确定。 |
| 产品咨询 | Syd Labs在国内只通过代理商销售其产品，不做直销。终端用户咨询价格请联系Syd Labs中国代理商。 关于Syd Labs产品如果有任何技术或其它问题，欢迎随时联系Syd Labs国内市场推广合作伙伴：武汉多找找科技有限公司，企业微信：duozhaozhao2024 联系电话：18162581039（龙经理） |
| 应用详情 | ELISA，流式细胞术（FC），中和（neutralization），功能测定如生物分析 PK 和 ADA 测定，以及那些用于研究受人CD3e蛋白影响的生物学途径的测定。 |
文献
抗人CD3e单克隆抗体，体内实验级重组，小鼠IgG2a Kappa (克隆号12F6)（In Vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa，货号：PA007387.m2a Syd Labs）
12F6 抗体可与 OKT3 抗体竞争结合人 T 细胞，且比 OKT3 具有更高效的 T 细胞抑制与激活特性。12F6 抗体常用于研究 T 细胞功能、调控 T 细胞的激活与抑制、预防急性同种异体移植排斥反应以及治疗自身免疫性糖尿病。
抗人CD3e单抗(12F6)部分引用文献:
1. Bispecific antibodies cross-link and redirect CD8+ T cells to kill cytomegalovirus-infected cells
Ali, A., et al. Sci Adv. 2024 Mar 1;10(9):eady2092. PMID: 38442054
“A bispecific antibody utilizing the anti-CD3 antibody (clone 12F6) was constructed to engage T cells in NOD/SCID mice infected with CMV. …In vivo studies demonstrated a significant reduction in viral load in treated mice. …Flow cytometry confirmed CD8+ T cell activation in spleen and liver tissues, showing enhanced T cell-mediated cytotoxicity against CMV-infected cells, while control groups receiving isotype antibodies exhibited no significant viral clearance.”
2. Novel humanized anti-CD3 antibodies induce a predominantly immunoregulatory profile in human peripheral blood mononuclear cells
Li, J., et al. Immunol Lett. 2009 Aug 30;125(2):75-84. PMID: 19410632
“The humanized anti-CD3 antibody (12F6) was administered to humanized mice to evaluate its immunoregulatory effects. …Treatment with 12F6 significantly reduced T cell proliferation in peripheral blood and increased IL-10 while decreasing IFN-γ levels. …Flow cytometry on blood samples confirmed in vivo T cell depletion, demonstrating that 12F6 (anti-human CD3e antibody) induces a regulatory T cell phenotype.”
3. Phase I trial of a humanized, Fc receptor nonbinding anti-CD3 antibody, hu12F6mu in patients receiving renal allografts
Sandborn, W. J., et al. MAbs. 2010 Jul-Aug;2(4):444-55. PMID: 20564327
“The hu12F6mu antibody, a humanized version of the 12F6 antibody (anti-human CD3e antibody), was administered to renal allograft patients to prevent rejection. …Flow cytometry confirmed reduced CD3+ T cell counts in peripheral blood within 48 hours. …The trial assessed the safety profile of hu12F6mu in a dose-escalation study, with no significant cytokine release syndrome reported in the low-dose cohort.”
4. Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions
Li, B., et al. Immunology. 2005 Dec;116(4):487-98. PMID: 16014509
“A humanized anti-CD3 monoclonal antibody (12F6) was tested in NOD/SCID mice reconstituted with human PBMCs. …The 12F6 antibody (anti-human CD3e antibody) effectively suppressed T cell-mediated graft-versus-host disease in vivo. …Flow cytometry showed a significant reduction in CD3+ T cells in spleen samples and a shift toward regulatory T cell populations, with no significant cytokine release observed.”
5. Humanization of the murine anti-human CD3 monoclonal antibody OKT3
Adair, J. R., et al. Hum Antibodies Hybridomas. 1994;5(1-2):41-7. PMID: 8006592
“The 12F6 antibody (anti-human CD3e antibody) was used as a comparator to assess the efficacy of humanized OKT3 in a humanized mouse model. …Both humanized OKT3 and 12F6 were administered to evaluate T cell suppression, showing comparable CD3+ T cell depletion via flow cytometry. …In vivo experiments demonstrated reduced graft rejection in treated animals, confirming the immunosuppressive properties of both antibodies.”